BIOSS

Centre for Biological Signalling Studies

News

Exploring Cutting-Edge Biological Signalling Research in Freiburg

The Lord Mayor of Freiburg, Martin Horn, recently embarked on a visit to the renowned Signalhaus Freiburg, a symbol of research excellence housing the Clusters

March 22, 2024 No Comments

2 new DFG research units at the University of Freiburg

The research unit “UcarE – Urothelial Carcinoma Epigenetics” aims to investigate the biological mechanisms that underlie bladder cancer. The unit’s spokesman is Prof Dr Ian

September 22, 2023 No Comments

First joint Open Day of CIBSS and BIOSS

In June, the two Freiburg signalling research centres CIBSS and BIOSS gave visitors insights into current research topics.

Glowing molecules and the memories of plants:

July 28, 2023 No Comments

Events

Thorsten Hugel

Institute of Physical Chemistry, University of Freiburg
Professor

+49 761 203 6192

thorsten.hugel@physchem.uni-freiburg.de

https://www.singlemolecule.uni-freiburg.de/group/hugel

Curriculum vitae

We use single molecule methods to investigate molecular machines and signaling in biological systems. We focus on understanding complex dynamic biological processes in and out of equilibrium. In addition, we probe the interaction between proteins and lipid bilayers. The core methods are single molecule multi-colour FRET and single molecule force spectroscopy.

10 selected publications

A blind benchmark of analysis tools to infer kinetic rate constants from single-molecule FRET trajectories. Götz M, Barth A, Bohr SS, Börner R, Chen J, Cordes T, Erie DA, Gebhardt C, Hadzic MCAS, Hamilton GL, Hatzakis NS, Hugel T, Kisley L, Lamb DC, de Lannoy C, Mahn C, Dunukara D, de Ridder D, Sanabria H, Schimpf J, Seidel CAM, Sigel RKO, Sletfjerding MB, Thomsen J, Vollmar L, Wanninger S, Weninger KR, Xu P, Schmid S. Nat Commun. 2022 Sep 14;13(1):5402.
Angle-dependent strength of a single chemical bond by stereographic force spectroscopy. Cai W, Bullerjahn JT, Lallemang M, Kroy K, Balzer BN, Hugel T. Chem Sci. 2022 Apr 26;13(19):5734-5740.
Nanosecond structural dynamics of the chaperone Hsp90 Sohmen B, Beck C, Seydel T, Hoffmann I, Hermann B, Nüesch M, Grimaldo M, Schreiber F, Wolf S, Roosen-RungeF, Hugel T (2021) arXiv, arxiv.org/abs/2110.10483.
Hierarchical dynamics in allostery following ATP hydrolysis monitored by single molecule FRET measurements and MD simulations. Wolf S, Sohmen B, Hellenkamp B, Thurn J, Stock G, Hugel T (2021). Chem Sci. 12(9):3350-3359.
Controlling protein function by fine-tuning conformational flexibility. Schmid S, Hugel T (2020). Elife. 9:e57180.
Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study. Hellenkamp B, Schmid S, et int., Hugel T (2018). Nat Methods. 15, 669.
Multidomain structure and correlated dynamics determined by self-consistent FRET networks. Hellenkamp B, Wortmann P, Kandzia F, Zacharias M, Hugel T (2017). Nat Methods. 14(2):174-180.
Folding and assembly of the large molecular machine Hsp90 studied in single-molecule experiments. Jahn M, Buchner J, Hugel T, Rief M (2016). Proc Natl Acad Sci U S A. 113(5):1232-7.
Four-colour FRET reveals directionality in the Hsp90 multicomponent machinery. Ratzke C, Hellenkamp B, Hugel T (2014). Nat Commun. 5:4192.
The large conformational changes of Hsp90 are only weakly coupled to ATP hydrolysis. Mickler M, Hessling M, Ratzke C, Buchner J, Hugel T (2009). Nat Struct Mol Biol.16(3):281-6.