Cluster of Excellence –
University of Freiburg

Design of a human rhinovirus-14 3C protease-inducible caspase-3

Wagner HJ, Weber W

Molecules 2019, 24, 1945; doi:10.3390/molecules24101945

Molecules                 online article

Here, we describe design strategies for the generation of a human rhinovirus-14 (HRV14) 3C protease-inducible caspase-3 (CASP3). We use our engineered CASP3 to characterize the effect of aspartate mutations on enzymatic activity. Besides the identification of mutations that render the enzyme inactive, we find the CASP3-D192E mutant (aspartate-to-glutamate exchange at position 192) to be inaccessible for 3C protease-mediated cleavage.