Cluster of Excellence –
University of Freiburg

Prof. Dr. Nils Wiedemann

Prof. Dr. Nils Wiedemann

Institute for Biochemistry and Molecular Biology
University of Freiburg

+49 761 203 5280

Biogenesis of mitochondrial membranes:

The mitochondrial membranes are crucial for oxidative phosphorylation and cell survival. Most of the mitochondrial membrane proteins and lipids are synthesized in the cytosol and the endoplasmic reticulum (ER) respectively. We analyze the ER-mitochondria encounter structure, which forms an inter-organellar connection, and the protein translocases in the inner and outer membrane required for the import of membrane proteins and lipids.

 

10 selected publications:

  • Membrane protein insertion through a mitochondrial β-barrel gate.
    Höhr AIC, Lindau C, Wirth C, Qiu J, Stroud DA, Kutik S, Guiard B, Hunte C, Becker T, Pfanner N, Wiedemann N (2018).
    Science 359, eaah6834.
  • Definition of a High-Confidence Mitochondrial Proteome at Quantitative Scale.
    Morgenstern M, Stiller SB, Lübbert P, Peikert CD, Dannenmeier S, Drepper F, Weill U, Höß P, Feuerstein R, Gebert M, Bohnert M, van der Laan M, Schuldiner M, Schütze C, Oeljeklaus S, Pfanner N, Wiedemann N*, Warscheid B* (2017).
    Cell Rep. 19, 2836–2852. (* corresponding)
  • Mitochondrial OXA translocase plays a major role in biogenesis of inner-membrane proteins.
    Stiller SB, Höpker J, Oeljeklaus S, Schütze C, Schrempp SG, Vent-Schmidt J, Horvath SE, Frazier AE, Gebert N, van der Laan M, Bohnert M, Warscheid B, Pfanner N, Wiedemann N (2016).
    Cell Metabolism 23, 901–908.
  • Sam37 is crucial for formation of the mitochondrial TOM-SAM supercomplex, thereby promoting β-barrel biogenesis.
    Wenz L-S, Ellenrieder L, Qiu J, Bohnert M, Zufall N, van der Laan M, Pfanner N*, Wiedemann N*, Becker T (2015).
    J Cell Biol 210, 1047–1054. (* corresponding)
  • Molecular architecture of the active mitochondrial protein gate.
    Shiota T, Imai K, Qiu J, Hewitt VL, Tan K, Shen H-H, Sakiyama N, Fukasawa Y, Hayat S, Kamiya M, Elofsson A, Tomii K, Horton P, Wiedemann N, Pfanner N, Lithgow T, Endo T (2015).
    Science 349, 1544–1548.
  • Coupling of Mitochondrial Import and Export Translocases by Receptor-Mediated Supercomplex Formation.
    Qiu J, Wenz L-S, Zerbes RM, Oeljeklaus S, Bohnert M, Stroud DA, Wirth C, Ellenrieder L, Thornton N, Kutik S, Wiese S, Schulze-Specking A, Zufall N, Chacinska A, Guiard B, Hunte C, Warscheid B, van der Laan M, Pfanner N*, Wiedemann N*, Becker T (2013).
    Cell 154, 596–608. (* corresponding)
  • Dual Function of Sdh3 in the Respiratory Chain and TIM22 Protein Translocase of the Mitochondrial Inner Membrane.
    Gebert N, Gebert M, Oeljeklaus S, Malsburg von der K, Stroud DA, Kulawiak B, Wirth C, Zahedi RP, Dolezal P, Wiese S, Simon O, Schulze-Specking A, Truscott KN, Sickmann A, Rehling P, Guiard B, Hunte C, Warscheid B, van der Laan M, Pfanner N, Wiedemann N (2011).
    Mol Cell 44, 811–818.
  • Composition and topology of the endoplasmic reticulum-mitochondria encounter structure.
    Stroud DA, Oeljeklaus S, Wiese S, Bohnert M, Lewandrowski U, Sickmann A, Guiard B, van der Laan M, Warscheid B, Wiedemann N (2011).
    J Mol Biol 413, 743–750.
  • Dissecting membrane insertion of mitochondrial β-barrel proteins.
    Kutik S, Stojanovski D, Becker L, Becker T, Meinecke M, Krüger V, Prinz C, Meisinger C, Guiard B, Wagner R, Pfanner N and Wiedemann N (2008).
    Cell 132, 1011-1024.
  • Machinery for protein sorting and assembly in the mitochondrial outer membrane.
    Wiedemann N, Kozjak V, Chacinska A, Schönfisch B, Rospert S, Ryan MT, Pfanner N and Meisinger C (2003).
    Nature 424, 565-571.