The diagnostic tool of the Freiburg iGEM Team published
The goal of the iGEM Team 2015 was the development of a diagnostic method that quickly and easily tests various diseases at the same time. To this end, they combined in vitro expression systems with label-free microfluidic readouts for detection of antibodies from blood sera.
Already in 2015, the team received for their project, The DiaCHIP, a gold medal at the iGEM (international Genetically Engineered Machine) competition in Boston, USA (http://www.pr.uni-freiburg.de/pm/2015/pm.2015-09-30.135)
Now, the Freiburg iGEM2015 Team completed the concept of the system with the recent article Multiplexed antibody detection from blood sera by immobilization of in vitro expressed antigens and label-free readout via imaging reflectometric interferometry (iRIf), in the journal Biosensors and Bioelectronics.
They successfully detected the presence of different antibodies in blood sera using an antigen microarray produced from DNA samples by an optimized in vitro expression mix.
This project was made possible through the generous funding from the BIOSS Centre for Biological Signalling Studies and several departments (Biology, Chemistry, and Medicine).
Multiplexed antibody detection from blood sera by immobilization of in vitro expressed antigens and label-free readout via imaging reflectometric interferometry (iRIf)
Bender J, Bognar S, Camagna M, Donauer JAM, Eble JW, Emig R, Fischer S, Jesser R, Keilholz L, Kokotek DMU, Neumann J, Nicklaus S, Oude Weernink RRQPT, Stühn LG, Wössner N, Krämer SD, Schwenk P, Gensch N, Roth G, Ulbrich MH.
Biosens Bioelectron. 2018 May 12;115:97-103