Centre for Biological Signalling Studies

Dr. Matthias Meier

Dr. Matthias Meier

IMTEK, University of Freiburg

+49 761 203 73241

Microfluidic Large Scale Integration and Bioengineering

My research group is focusing on the development and usage of large-scale microfluidic integration platforms for screening and characterization of biomolecular interactions within the regulatory network of the cell metabolism. In order to obtain a systems biology perspective we work on three different biological levels, i.e. the molecular, cellular and organism level. On the molecular level we envision the construction of protein interaction maps based on thermodynamics, kinetics structural parameters rather than only on binary interaction information. On the cellular and organismic level we aim to characterize the cell metabolism upon changing cell environments and simulate with help of microengineering approaches real world conditions. We emphasize our work on post-translational modifications and epigenetic factors acting on different timescales. Bringing together broad experimental protein interaction information from various biological levels and different biophysical angles we integrate information about the regulation of cell metabolism under various conditions including disease states in human and crop plants.


10 selected publications

  • Kinetic engineering of a Fluorescence Riboswitch for Glycine.
    Ketterer S, Schneider N, Meier M.
    Nucleic Acids Res 2016; 44(12):5983-92
  • Systematic reconstruction of binding and stability landscapes of the fluorogenic aptamer spinach.
    Ketterer S, Fuchs D, Weber W, Meier M.
    Nucleic Acids Res. 2015 Oct 30;43(19):9564-72
  • A versatile and simple enzyme-free cloning approach for synthetic biology
    Beyer HM, Gonschorek P, Meier M, Weber W, Zurbriggen M.
    PlosOne 2015, 10(9): e0137652
  • Microfluidic Proximity Ligation Assay for Profiling Signaling Networks with Single-Cell Resolution.
    Blazek M, Roth G, Zengerle R, Meier M.
    Methods Mol Biol. 2015;1346:169-84.
  • Analysis of fast protein phosphorylation kinetics in single cells on a microfluidic chip.
    Blazek M, Santisteban TS, Zengerle R, Meier M.
    Lab Chip. 2015 Feb 7;15(3):726-34
  • Transcription factor sensor system for parallel quantification of metabolites on-chip.
    Ketterer S, Hövermann D, Guebeli RJ, Bartels-Burgahn F, Riewe D, Altmann T, Zurbriggen MD, Junker B, Weber W, Meier M.
    Anal Chem. 2014 Dec 16;86(24):12152-8.
  • Design and validation of DNA libraries for multiplexing proximity ligation assays.
    Gobet N, Ketterer S, Meier M.
    PLoS One. 2014 Nov 11;9(11):e112629
  • Proximity ligation assay for high-content profiling of cell signaling pathways on a microfluidic chip.
    Blazek M, Betz C, Hall MN, Reth M, Zengerle R, Meier M.
    Mol Cell Proteomics. 2013 Dec;12(12):3898-907
  • Proteome-wide protein interaction measurements of bacterial proteins of unknown function.
    Meier M, Sit RV, Quake SR.
    Proc Natl Acad Sci U S A. 2013 Jan 8;110(2):477-82
  • Systematic reconstruction of RNA functional motifs with high-throughput microfluidics.
    Martin L, Meier M, Lyons SM, Sit RV, Marzluff WF, Quake SR, Chang HY.
    Nat Methods. 2012 Dec;9(12):1192-4