Centre for Biological Signalling Studies

Dr. Günter Roth

Dr. Günter Roth

ZBSA, Microarray Copying, Universität Freiburg

+49 761 203 97167


Laser-Scanning-Microscopy, Epi-Fluorescence-Microscopy, FRET and FRAP, Atomic Force microscopy, Scanning electron microscopy with EDY, WDY, SEM and TEM, Ellipsometry, Anisotropy-Spectroscopy, Fluorescence Correlation Spectroscopy, Surface plasmon resonance (Biacore), Reflectometric Interference Spectroscopy, Isoelectric Focussing, Electrophoresis, Single molecule detection

Cleanroom and semiconductors, micro contact printing, CVD-methods, laser physics, rotation-polarisation-meassurements, electron spin measurements

Solid phase synthesis (peptides, lipopeptides drug-like compounds), generation of chemical libraries by split and mix synthesis, silanization and modification of surfaces and solid phases (glass, plastics, beads and membranes), chemical modification and ligation of biomolecules, molecular tagging (his, myc, biotin, fluorophores)

Microarray generation (DNA, peptides, proteins, drug-like compounds, antibodies), cell penetrating peptides, electroporation, transfection, T-cell signalling, high throughput screening of peptide libraries, activation of B- and T-cells by PAMPs, ligand receptor analysis, classic immuno- and cell cultre methods.


10 selected publications

  • Low-Volume Label-Free Detection of Molecule-Protein Interactions on Microarrays by Imaging Reflectometric Interferometry
    Burger J, Rath C, Woehrle J, Meyer PA, Ammar NB, Kilb N, Brandstetter T, Pröll F, Proll G, Urban G, Roth G
    Journal of Laboratory Automation 2016, DOI: 10.1177/2211068216657512
  • Semi-contact-writing of polymer molds for prototyping PDMS chips with low surface roughness, sharp edges and locally varying channel heights
    Gutzweiler L, Stumpf F, Tanguy L, Roth G, Koltay P, Zengerle R, Riegger L (2016).
    J Micromech Microeng. 26(4):45018 – 45027
  • A peptide-functionalized polymer as a minimal scaffold protein to enhance cluster formation in early T cell signal transduction.
    Witsenburg JJ, Sinzinger MD, Stoevesandt O, Ruttekolk IR, Roth G, Adjobo-Hermans MJ, Brock R (2015)
    Chembiochem. 16(4):602-10.
  • Single-cell PCR of genomic DNA enabled by automated single-cell printing for cell isolation
    Stumpf F, Schoendube J, Gross A, Rath C, Niekrawietz S, Koltay P, Roth G (2015).
    Biosens Bioelectron; DOI 10.1016/j.bios.2015.03.008
  • Protein microarray generation by in situ protein expression from template DNA.
    Kilb N, Burger J, Roth G (2014)
    Engineering in Life Sciences. 14(4): 352-364
  • A quantitative assessment of costimulation and phosphatase activity on microclusters in early T cell signaling.
    Witsenburg JJ, Glauner H, Müller JP, Groenewoud JM, Roth G, Böhmer FD, Adjobo-Hermans MJ, Brock R (2013)
    PLoS One. 8(10):e79277
  • Multiplex genotyping of KRAS point mutations in tumor cell DNA by allele-specific real-time PCR on a centrifugal microfluidic disk segment.
    Strohmeier O, Laßmann S, Riedel B, Mark D, Roth G, Werner M et al (2013).
    Microchimica Acta. 181(13-14):1681-1688
  • Mediator probe PCR: a novel approach for detection of real-time PCR based on label-free primary probes and standardized secondary universal fluorogenic reporters.
    Faltin B, Wadle S, Roth G, Zengerle R, von Stetten F (2012).
    Clin Chem. 58(11):1546-56
  • Universal protocol for grafting PCR primers onto various lab-on-a-chip substrates for solid-phase PCR.
    Hoffmann J, Hin S, von Stetten F, Zengerle R, Roth G (2013).
    RSC Advances 2(9):3885-3889
  • Solid-phase PCR in a picowell array for immobilizing and arraying 100000 PCR products to a microscope slide.
    Hoffmann J, Trotter M, von Stetten F, Zengerle R, Roth G (2012).<br/> Lab on a Chip. 12(17):3049-3054