Centre for Biological Signalling Studies

Phosphorylation of Beclin-1 by BCR-ABL suppresses autophagy in chronic myeloid leukemia

Yu C, Gorantla SP, Müller-Rudorf A, Müller TA, Kreutmair S, Albers C, Jakob L, Lippert LJ, Yue Z, Engelhardt M, Follo M, Zeiser R, Huber TB, Duyster J, Illert AL.

Haematologica. 2019 Aug 8. [Epub ahead of print]

Haematologica.        online article

Autophagy is a genetically regulated process of adaptation to metabolic stress and was recently shown to be involved in the treatment response of chronic myeloid leukemia. However, in vivo data are limited and the molecular mechanism of autophagy regulators in the process of leukemogenesis is not completely understood. Here we show that Beclin-1 knockdown, but not Atg5 deletion in a murine chronic myeloid leukemia model leads to a reduced leukemic burden and results in a significantly prolonged median survival of targeted mice. Further analyses of murine cell lines and primary patient material indicate that active BCR-ABL directly interacts with Beclin-1 and phosphorylates its tyrosine residues 233 and 352, resulting in autophagy suppression. By using phosphorylation-deficient and phosphorylation-mimic mutants, we identify BCR-ABL induced Beclin-1 phosphorylation as a crucial mechanism for Beclin-1 complex formation: Interaction analyses exhibit diminished binding of the positive autophagy regulators UVRAG, VPS15, ATG14 and VPS34 and enhanced binding of the negative regulator Rubicon to BCR-ABL-phosphorylated Beclin-1. Taken together, our findings show interaction of BCR-ABL and Beclin-1 thereby highlighting the importance of Beclin-1-mediated autophagy in BCR-ABL+ cells.