Human ORF clones
All clones from the human ORFeome V5.1 collection (Open Biosystems) are available from the BIOSS Toolbox upon request only for research members of the University of Freiburg and the University Medical Center Freiburg.
- Each human ORF clone comes from a mini-pool of PCR-amplified pDONR223 vector (Invitrogen), therefore contaminations or mutations might have taken place in the process. Bacterial plates are not verified before distribution! Please double-check the sequence of cDNA by sequencing or colony PCR before you transfer the sequence into the desired vector. (Sequencing with T7 or M13for and rev primer is possible.)
- The pDONR223 vector has a spectinomycin resistance! We recommend a concentration of Spectinomycin in media of 50 µg/ml.
- cDNA clones can be transferred easily using Gateway® LR Clonase (Gateway ® LR Clonase ® II enzyme mix , Cat no. 11791-020) in any Gateway® compatible vector. PCR amplification of cDNA is also possible.
Purchase Order Regulation:
As a non-profit repository, the Toolbox properly stores and maintains the human ORFeome collection in the BIOSS Signalhaus and thereby makes it available for academic and non-profit laboratories in Freiburg.
- On request the Toolbox team will provide human ORF clones as bacterial plate.
- To support operation of the Toolbox Repository and to maintain the high quality standard of our work we charge a fee for hORFeome clones. The fee is used to cover consumables and operating cost. (The transfer of material contemplated herein shall not be considered as a "sale of goods" for any purposes.) Any request of material will be covered under a Toolbox-MTA between the RECIPIENT and the BIOSS Toolbox.
Members of the University of Freiburg & the University Medical Center Freiburg