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You are here: Toolbox » Plasmid Shop » pAkt-PH-mCherry [879]

pAkt-PH-mCherry [879]

pAkt-PH-mCherry [879]

NOTE: this plasmid is available for members of the University of Freiburg only!

plasmid map

Plasmid Information

Toolbox-No.	879
Functional Description	PIP3 sensor, plasma membrane marker
Sequence of plasmid	pAkt-PH-mCherry show sequence
GCK-file (v3.0)	pAkt-PH-mCherry.gcc
GenBank-File	pAkt-PH-mCherry.gb
Key elements of plasmid	pAkt-PH-mCherryx.pdf
Verified via	digestThis plasmid was verified with restriction digest. Toolbox does not guarantee the correctness of the sequence. sequencingInsert and flanking restriction site were sequenced by the Toolbox. Detailed information can be found in the GCK-file (see Construct/General Info) and in the GenBank-file (Comment or Info).

Protein Information

Complete Protein Insert	Akt-PH-mCherry show sequence
Protein of Interest	Akt-PH domain show sequence
Species of Protein	Homo sapiens
Fusions or Tags - C terminal	mCherry

Vector Information

Vector name	pmCherry-N1
Type of vector/expressed in	mammalian cells
Bacterial resistances	kanamycin
Selectable Marker	Neomycin
High or Low copy	High
Growth in standard E. coli	Yes
Growth conditions	37°C
Plasmid provided in	DH10B

Cloning Information

Insert size (bp)	444
Cloning Sites 5' - 3'	KpnI - BamHI

Additional Information

Principle Investigator	Dr. Winfried Römer (⇒ www)
Protected Sequences	Backbone, mCherry
Vendor Company	Clontech (⇒ www)
Vendor Order Number	632523

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pAkt-PH-mCherry [879]
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