pD-nLynYN173 [783]
NOTE: this plasmid is available for members of
the University of Freiburg only!
Plasmid Information
| Toolbox-No. |
783 |
| Functional Description |
fusion construct of murine N-terminal tyrosine kinase Lyn (aa1-24) with the EYFP variant YN173 for using in BiFC (Bifluorescence Complementation) experiments
|
| Sequence of plasmid |
pD-nLynYN173
show sequence
|
| GCK-file (v3.0) |
pD-nLynYN173.gcs |
| GenBank-File |
pD-nLynYN173.gb |
| Verified via |
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Protein Information
| Protein of Interest |
nLyn-L1-YN173
show sequence
|
| Full length or fragment |
Full Length |
| Fusions or Tags - C terminal |
YN=YN173 =EYFP(1-173aa) |
Vector Information
| Vector name |
pD alias pRmHA-3 |
| Type of vector/expressed in |
Drosophila cells, expressed in S2 cells |
| Bacterial resistances |
ampicilllin |
| High or Low copy |
High |
| Growth in standard E. coli |
Yes |
| Growth conditions |
37°C |
| Plasmid provided in |
DH10B |
Cloning Information
| Insert size (bp) |
615 |
| Cloning Sites 5' - 3' |
EcoRI NcoI - AccI, HincII, SalI |
Additional Information
| Principle Investigator |
Dr. Jianying YANG (⇒ www) |
| Protected Sequences |
YFP |
Your Recent History
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