CoralHue pKaede-MC1 
NOTE: this plasmid is available for members of the University of Freiburg only!
|Functional Description||CoralHue TM Kaede emits bright green fluorescence that can be irreversibly converted to red fluorescence. The red fluorescence is comparable in intensity to the green fluorescence and is stable under usual aerobic conditions. This green-to-red photoconversion is highly sensitive to irradiation with UV or violet light (350-410 nm).
The excitation lights used to elicit red and green
fluorescence do not induce the photoconversion. This
property provides a simple and powerful technique for
regional optical marking ( see: http://www.mblintl.com/product/am-v0012). CoralHue TM Kaede protein forms a tetramer.
Genes of interest can be cloned into the MCS and will be expressed as fusions to the C-terminus of Kaede. (source: MBL)
|Sequence of plasmid||CoralHue pKaede-MC1 show sequence|
|GCK-file (v3.0)||CoralHue pKaede-MC1(MBL).gcs|
digestThis plasmid was verified with restriction digest. Toolbox does not guarantee the correctness of the sequence.
|Protein of Interest||Kaede show sequence|
|Species of Protein||synthetic construct|
|Full length or fragment||Full Length|
|Type of vector/expressed in||mammalian cells|
|High or Low copy||High|
|Growth in standard E. coli||Yes|
|Plasmid provided in||DH10B|
|Insert size (bp)||678|
|Cloning Sites 5' - 3'||NheI - BamHI, EcoRI, XhoI, AvaI, HindIII (after STOP codon: NotI, XbaI, MfeI, HpaI)|
|Protected Sequences||Backbone, CoralHue Kaede|
|Vendor Company||MBL (⇒ www)|
|Vendor Order Number||AM-V0012|