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You are here: Toolbox » Plasmid Shop » CoralHue pKaede-MC1 [484]

CoralHue pKaede-MC1 [484]

CoralHue pKaede-MC1 [484]
Manufacturer: MBL
NOTE: this plasmid is available for members of the University of Freiburg only!
plasmid map

Plasmid Information

Toolbox-No. 484
Functional Description CoralHue TM Kaede emits bright green fluorescence that can be irreversibly converted to red fluorescence. The red fluorescence is comparable in intensity to the green fluorescence and is stable under usual aerobic conditions. This green-to-red photoconversion is highly sensitive to irradiation with UV or violet light (350-410 nm).
The excitation lights used to elicit red and green
fluorescence do not induce the photoconversion. This
property provides a simple and powerful technique for
regional optical marking ( see: http://www.mblintl.com/product/am-v0012). CoralHue TM Kaede protein forms a tetramer.
Genes of interest can be cloned into the MCS and will be expressed as fusions to the C-terminus of Kaede. (source: MBL)
Sequence of plasmid CoralHue pKaede-MC1 show sequence
GCK-file (v3.0) CoralHue pKaede-MC1(MBL).gcs
GenBank-File CoralHue pKaede-MC1(MBL).gb
Verified via

Protein Information

Protein of Interest Kaede show sequence
Species of Protein synthetic construct
Full length or fragment Full Length

Vector Information

Type of vector/expressed in mammalian cells
Bacterial resistances kanamycin
Selectable Marker Neomycin
High or Low copy High
Growth in standard E. coli Yes
Growth conditions 37°C
Plasmid provided in DH10B

Cloning Information

Insert size (bp) 678
Cloning Sites 5' - 3' NheI - BamHI, EcoRI, XhoI, AvaI, HindIII (after STOP codon: NotI, XbaI, MfeI, HpaI)

Additional Information

Protected Sequences Backbone, CoralHue Kaede
Vendor Company MBL (⇒ www)
Vendor Order Number AM-V0012
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