CoralHue pmAG1-MC1(MBL) [486]
NOTE: this plasmid is available for members of
the University of Freiburg only!
Plasmid Information
| Toolbox-No. |
486 |
| Functional Description |
The plasmid DNA encodes a monomeric version of the fluorescent protein “CoralHue™ Azami-Green (AG)”. AG was cloned from the stony coral whose Japanese name is “Azami-Sango”(adsorption/emission=492 /505 nm).
This expression plasmid is designed for insertion of a target gene downstream of the mAG1 sequence. (source: MBL; further information see MBL website: http://www.mblintl.com/product/am-v0032) |
| Sequence of plasmid |
CoralHue pmAG1-MC1(MBL)
show sequence
|
| GCK-file (v3.0) |
CoralHue pmAG1-MC1(MBL).gcs |
| GenBank-File |
CoralHue pmAG1-MC1(MBL).gb |
| Verified via |
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Protein Information
Vector Information
| Type of vector/expressed in |
mammalian cells |
| Bacterial resistances |
kanamycin |
| Selectable Marker |
Neomycin |
| High or Low copy |
High |
| Growth in standard E. coli |
Yes |
| Growth conditions |
37°C |
| Plasmid provided in |
DH10B |
Cloning Information
| Insert size (bp) |
678 |
| Cloning Sites 5' - 3' |
NheI - BamHI, EcoRI, XhoI, AvaI, HindIII (after STOP codon: NotI, XbaI) |
Additional Information
| Protected Sequences |
Backbone, mAG1 |
| Vendor Company |
MBL (⇒ www) |
| Vendor Order Number |
AM-V0032 |
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