CoralHue pmAG1-MC1(MBL) 
NOTE: this plasmid is available for members of the University of Freiburg only!
|Functional Description||The plasmid DNA encodes a monomeric version of the fluorescent protein “CoralHue™ Azami-Green (AG)”. AG was cloned from the stony coral whose Japanese name is “Azami-Sango”(adsorption/emission=492 /505 nm).
This expression plasmid is designed for insertion of a target gene downstream of the mAG1 sequence. (source: MBL; further information see MBL website: http://www.mblintl.com/product/am-v0032)
|Sequence of plasmid||CoralHue pmAG1-MC1(MBL) show sequence|
|GCK-file (v3.0)||CoralHue pmAG1-MC1(MBL).gcs|
digestThis plasmid was verified with restriction digest. Toolbox does not guarantee the correctness of the sequence.
|Protein of Interest||mAG1 (Azami-Green1 ) show sequence|
|Species of Protein||synthetic construct|
|Full length or fragment||Full Length|
|Type of vector/expressed in||mammalian cells|
|High or Low copy||High|
|Growth in standard E. coli||Yes|
|Plasmid provided in||DH10B|
|Insert size (bp)||678|
|Cloning Sites 5' - 3'||NheI - BamHI, EcoRI, XhoI, AvaI, HindIII (after STOP codon: NotI, XbaI)|
|Protected Sequences||Backbone, mAG1|
|Vendor Company||MBL (⇒ www)|
|Vendor Order Number||AM-V0032|