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You are here: Toolbox » Plasmid Shop » pLKO.1-neo_TRCN0000006803 [836]

pLKO.1-neo_TRCN0000006803 [836]

pLKO.1-neo_TRCN0000006803 [836]

Manufacturer:

Sigma-Aldrich

NOTE: this plasmid is available for members of the University of Freiburg only!

plasmid map

Plasmid Information

Toolbox-No.	836
Functional Description	FAP_TRC1 shRNA (clone ID NM_004460.2-879s1c1) containing lentiviral vector for silencing of the fibroblast activation protein (NM_004460), the vector backbone is derivative of the pLKO.1-puro with an exchange of puromycin cassette into neomycin resistance gene
Sequence of plasmid	pLKO.1-neo_TRCN0000006803 show sequence
GCK-file (v3.0)	pLKO.1-neo_TRCN0000006803.gcs
GenBank-File	pLKO.1-neo_TRCN0000006803.gb
Key elements of plasmid	pLKO.1-neo_TRCN0000006803x.pdf
Verified via	digestThis plasmid was verified with restriction digest. Toolbox does not guarantee the correctness of the sequence. sequencingInsert and flanking restriction site were sequenced by the Toolbox. Detailed information can be found in the GCK-file (see Construct/General Info) and in the GenBank-file (Comment or Info).

Vector Information

Vector name	pLKO.1-neo
Type of vector/expressed in	lentiviral, mammalian cells
Bacterial resistances	ampicillin, kanamycin
Selectable Marker	Neomycin
High or Low copy	High
Growth conditions	37°C
Plasmid provided in	DH10B

Additional Information

Protected Sequences	Backbone
Comments	The growth of bacterias with kanamycin is not recommended, if it is necessary please incubate the cultur up to 48h !
Vendor Company	Sigma-Aldrich (⇒ www)

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pLKO.1-neo_TRCN0000006803 [836]
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