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You are here: Toolbox » Plasmid Shop » CoralHue pKiKGR1-MC1 [485]

CoralHue pKiKGR1-MC1 [485]

CoralHue pKiKGR1-MC1 [485]

Manufacturer:

MBL

NOTE: this plasmid is available for members of the University of Freiburg only!

plasmid map

Plasmid Information

Toolbox-No.	485
Functional Description	KikGR1 emits bright green fluorescence that can be irreversibly converted to red fluorescence. The red fluorescence is comparable in intensity to the green fluorescence and is stable under usual aerobic conditions. This green-to-red photoconversion is highly sensitive to irradiation with UV or violet light (350-420 nm). Maximal illumination results in a robust increase in the ratio of red-to-green signal. KikGR1 is several-fold brighter and more efficiently photoconverted than our other Green-red photo- converting protein, CoralHueTM Kaede. CoralHue pKiKGR1 forms a tetramer(Further information see: http://www.mblintl.com/product/am-v0083). Genes of interest can be cloned into the MCS and will be expressed as fusions to the C-terminus of KikGR1.(source: MBL)
Sequence of plasmid	CoralHue pKiKGR1-MC1 show sequence
GCK-file (v3.0)	CoralHue pKiKGR1-MC1(MBL).gcs
GenBank-File	CoralHue pKiKGR1-MC1(MBL).gb
Verified via	digestThis plasmid was verified with restriction digest. Toolbox does not guarantee the correctness of the sequence. sequencingInsert and flanking restriction site were sequenced by the Toolbox. Detailed information can be found in the GCK-file (see Construct/General Info) and in the GenBank-file (Comment or Info).

Protein Information

Protein of Interest	Kikume Green-Red1 show sequence
Species of Protein	synthetic construct
Full length or fragment	Full Length

Vector Information

Type of vector/expressed in	mammalian cells
Bacterial resistances	kanamycin
Selectable Marker	Neomycin
High or Low copy	High
Growth in standard E. coli	Yes
Growth conditions	37°C
Plasmid provided in	DH10B

Cloning Information

Insert size (bp)	747
Cloning Sites 5' - 3'	NheI - BamHI, EcoRI, XhoI, AvaI, HindIII, XbaI (after STOP codon: NotI, MfeI, HpaI, HincII)

Additional Information

Protected Sequences	Backbone, Kikume Green-Red1
Vendor Company	MBL (⇒ www)
Vendor Order Number	AM-V0082

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CoralHue pKiKGR1-MC1 [485]
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