CoralHue pmKO1-S1 [488]
NOTE: this plasmid is available for members of
the University of Freiburg only!
Plasmid Information
| Toolbox-No. |
488 |
| Functional Description |
monomeric version of the fluorescent protein CoralHue TM Kusabira Orange (KO); KO has been cloned from the stony coral "Kusabira-ishi"; absorbtion/emission=548/561 nm;
mKO can be used to label proteins or subcellular structures or for FRET analysis.
pmKO-S1 is not an expression vector. To express
mKO1 in cells, the cDNA must be transferred to an
appropriate expression vector. (source: MBL) |
| Sequence of plasmid |
CoralHue pmKO1-S1
show sequence
|
| GCK-file (v3.0) |
CoralHue pmKO1-S1(MBL).gcs |
| GenBank-File |
CoralHue pmKO1-S1(MBL).gb |
| Verified via |
|
Protein Information
Vector Information
| Type of vector/expressed in |
bacterias, E.coli; non expression vector! |
| Bacterial resistances |
ampicillin |
| High or Low copy |
High |
| Growth in standard E. coli |
Yes |
| Growth conditions |
37°C |
| Plasmid provided in |
DH10B |
Cloning Information
| Insert size (bp) |
657 |
| Cloning Sites 5' - 3' |
NcoI, BamHI, SmaI, AvaI, EcoRI - HIndIII |
Additional Information
| Protected Sequences |
Backbone, Kusabira Orange |
| Vendor Company |
MBL (⇒ www) |
| Vendor Order Number |
AM-V0051 |
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