pTimer-1 [715]
NOTE: this plasmid is available for members of
the University of Freiburg only!
Plasmid Information
| Toolbox-No. |
715 |
| Functional Description |
pTimer-1 is a promoterless vector that can be used to monitor transcription from different promoters and promoter/enhancer combinations inserted into the MCS located upstream of the DsRed1-E5 coding sequence.
Shortly after translation, DsRed1-E5 emits green light; its red fluorophore emerges later, hours after translation. Because of its predictable color shift, DsRed1-E5 acts as a timer, useful for monitoring changes in gene activity in living cells. pTimer-1 can be used to study the on-off activity of any cis-acting regulatory element cloned into the MCS upstream of DsRed1-E5.(source Clontech)
|
| Sequence of plasmid |
pTimer-1
show sequence
|
| GCK-file (v3.0) |
pTimer-1(Clontech).gcs |
| GenBank-File |
pTimer-1(Clontech).gb |
| Verified via |
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Protein Information
Vector Information
| Type of vector/expressed in |
bacterial, mammalian vector (without promoter) |
| Bacterial resistances |
kanamycin |
| Selectable Marker |
Neomycin |
| Growth in standard E. coli |
Yes |
| Growth conditions |
37°C |
| Plasmid provided in |
DH10B |
Additional Information
| Protected Sequences |
Backbone, dsRed1-E5 |
| Associated Publications |
pTimer-1 |
| Vendor Company |
Clontech (⇒ www) |
| Vendor Order Number |
632403 |
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