pMIG-Venus [115]
![pMIG-Venus [115]](images/products/product_info/default_plasmid.png "pMIG-Venus [115]")
Plasmid Information
| Toolbox-No. | 115 |
| Functional Description | Retroviral expression vector derives from pMIG; the GFP of the original IRES-GFP casette is exchanged with the encoding enhanced yellow fluorescent protein (venus). The vector allows cloning of genes of interest on the N-terminus of IRES for co-expression with venus protein in cells. |
| Sequence of plasmid | pMIG-Venus show sequence |
| GCK-file (v3.0) | pMIG-Venus.gcs |
| GenBank-File | pMIG-Venus.gb |
| Key elements of plasmid | pMIG-Venusx.pdf |
| Verified via |
Protein Information
| Protein of Interest | Venus show sequence |
| Species of Protein | synthetic construct |
| Relevant mutations | - |
Vector Information
| Type of vector/expressed in | mammalian cells |
| Bacterial resistances | ampicillin |
| High or Low copy | High |
| Growth in standard E. coli | Yes |
| Growth conditions | 37°C |
| Plasmid provided in | XL-1 Blue |
Cloning Information
| Insert size (bp) | - |
| Cloning Sites 5' - 3' | BglII, XhoI, HpaI, EcoRI |
Additional Information
| Principle Investigator | Dr. Hassan Jumaa (⇒ www) |
| Protected Sequences | Venus |
Related Plasmids
| Plasmids using this vector | pMIG-Venus-SLP65 |
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![pMIG-Venus [115]](images/products/mini/default_plasmid.png "pMIG-Venus [115]")