pGEMHE-XfA4-PAmCherry1 [509]
![pGEMHE-XfA4-PAmCherry1 [509]](images/products/product_info/default_plasmid.png "pGEMHE-XfA4-PAmCherry1 [509]")
NOTE: this plasmid is available for members of
the University of Freiburg only!
Plasmid Information
| Toolbox-No. | 509 |
| Functional Description | Chimeric modified CNG channel (cyclic nucleotide gated channel) fused with the photoswitchable PAmCherry1 (a derivative of mCherry, see Pubmed 19169259). The XfA4- CNNG-channel forms tetramers.
Plasmid can be used as reference for "subunit counting" in single molecule experiments. |
| Sequence of plasmid | pGEMHE-XfA4-PAmCherry1 show sequence |
| PubMed ID | 19169259 |
| GCK-file (v3.0) | pGEMHE-XfA4-PAmCherry1.gcs |
| GenBank-File | pGEMHE-XfA4-PAmCherry1.gb |
| Key elements of plasmid | pGEMHE-XfA4-PAmCherry1x.pdf |
| Verified via |
Protein Information
| Complete Protein Insert | XfA4 - PAmCherry1 show sequence |
| Protein of Interest | XfA4 show sequence |
| Species of Protein | chimeric |
| Full length or fragment | Full Length |
| Fusions or Tags - C terminal | Linker2, PAmCherry1(AY678264.1) |
Vector Information
| Vector name | pGEMHE |
| Type of vector/expressed in | Xenopus oocytes |
| Bacterial resistances | ampicillin |
| High or Low copy | Low |
| Growth in standard E. coli | Yes |
| Growth conditions | 37°C |
Cloning Information
| Insert size (bp) | 2763 |
| Cloning Sites 5' - 3' | SmaI, BamHI, SalI - HIndIII, SpeI, NotI |
Additional Information
| Protected Sequences | , PAmCherry1 |
| Associated Publications | Photoactivatable mCherry for high-resolution two-color fluorescence microscopy; Subach et al., 2009 |
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