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You are here: Toolbox » Plasmid Shop » pD Lifeact_eYFP [689]

pD Lifeact_eYFP [689]

pD Lifeact_eYFP [689]

plasmid map

Plasmid Information

Toolbox-No.	689
Functional Description	Drosophila vector with special linker Lifeact and enhanced yellow flourescent protein for generation of N-terminal fusion proteins.
Sequence of plasmid	pD Lifeact_eYFP show sequence
GCK-file (v3.0)	pD Lifeact_eYFP.gcs
GenBank-File	pD Lifeact_eYFP.gb
Key elements of plasmid	pD Lifeact_eYFPx.pdf
Verified via	digestThis plasmid was verified with restriction digest. Toolbox does not guarantee the correctness of the sequence. sequencingInsert and flanking restriction site were sequenced by the Toolbox. Detailed information can be found in the GCK-file (see Construct/General Info) and in the GenBank-file (Comment or Info).

Protein Information

Complete Protein Insert	eYFP show sequence
Protein of Interest	eYFP show sequence
Species of Protein	synthetic construct
Full length or fragment	Full Length
Fusions or Tags - N terminal	Lifeact= linker

Vector Information

Vector name	pD alias pRmHA-3
Type of vector/expressed in	Drosophila cells, expressed in S2 cells
Bacterial resistances	ampicillin
High or Low copy	High
Growth in standard E. coli	Yes
Growth conditions	37°C
Plasmid provided in	DH10B

Cloning Information

Cloning Sites 5' - 3'

EcoRI, NcoI

Additional Information

Principle Investigator	Dr. Jianying YANG (⇒ www)
Protected Sequences	Venus

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pD Lifeact_eYFP [689]
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