pKillerRed-mem [387]
NOTE: this plasmid is available for members of
the University of Freiburg only!
Plasmid Information
| Toolbox-No. |
387 |
| Functional Description |
Vector allows generation of fusions with protein of interest to the N-terminus of KillerRed. The fusion with MLS (membrane localization signal) of Neuromodulin targets KillerRed to cellular membranes . KillerRed is a phototoxic red fluorescent protein (source: Evrogen, see www.evrogen.com/products/KillerRed/KillerRed_Detailed_description.shtml). |
| Sequence of plasmid |
pKillerRed-mem
show sequence
|
| GCK-file (v3.0) |
pKillerRed-mem(Evrogen).gcs |
| GenBank-File |
pKillerRed-mem(Evrogen).gb |
| Verified via |
|
Protein Information
Vector Information
| Vector name |
pCMV_kan |
| Type of vector/expressed in |
mammalian cells |
| Bacterial resistances |
kanamycin |
| Selectable Marker |
Neomycin |
| High or Low copy |
High |
| Growth in standard E. coli |
Yes |
| Growth conditions |
37°C |
| Plasmid provided in |
DH10B |
Cloning Information
| Insert size (bp) |
764 |
| Cloning Sites 5' - 3' |
AfeI, XhoI, HindIII, EcoRI, SalI, SmaI, AccI, SacII, BamHI, AgeI - XbaI, NotI |
Additional Information
| Protected Sequences |
Backbone, KillerRed |
Your Recent History
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