NOTE: this plasmid is available for members of the University of Freiburg only!
|Functional Description||yeast two hybrid system shuttle vector, contains the Gal4 activation domain;
The hybrid protein is targeted to the yeast nucleus by nuclear localization sequences that have been addedto the AD sequence .
|Sequence of plasmid||pGAD424 show sequence|
digestThis plasmid was verified with restriction digest. Toolbox does not guarantee the correctness of the sequence.
|Protein of Interest||Gal4-AD show sequence|
|Full length or fragment||Fragment|
|Type of vector/expressed in||E. coli, S. cerevisiae|
|High or Low copy||High|
|Growth in standard E. coli||Yes|
|Plasmid provided in||DH10B|
|Cloning Sites 5' - 3'||see MCS|
|Vendor Company||Clontech (⇒ www)|
|Vendor Order Number||K1605-1|