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You are here: Toolbox » Plasmid Shop » pGAD424 [520]

pGAD424 [520]

pGAD424 [520]

Manufacturer:

Clontech

NOTE: this plasmid is available for members of the University of Freiburg only!

plasmid map

Plasmid Information

Toolbox-No.	520
Functional Description	yeast two hybrid system shuttle vector, contains the Gal4 activation domain; The hybrid protein is targeted to the yeast nucleus by nuclear localization sequences that have been addedto the AD sequence .
Sequence of plasmid	pGAD424 show sequence
PubMed ID	2547163
GCK-file (v3.0)	pGAD424(Clontech).gcs
GenBank-File	pGAD424(Clontech).gb
Verified via	digestThis plasmid was verified with restriction digest. Toolbox does not guarantee the correctness of the sequence. sequencingInsert and flanking restriction site were sequenced by the Toolbox. Detailed information can be found in the GCK-file (see Construct/General Info) and in the GenBank-file (Comment or Info).

Protein Information

Protein of Interest	Gal4-AD show sequence
Full length or fragment	Fragment

Vector Information

Type of vector/expressed in	E. coli, S. cerevisiae
Bacterial resistances	ampicillin
High or Low copy	High
Growth in standard E. coli	Yes
Growth conditions	37°C
Plasmid provided in	DH10B

Cloning Information

Cloning Sites 5' - 3'

see MCS

Additional Information

Protected Sequences	Backbone
Vendor Company	Clontech (⇒ www)
Vendor Order Number	K1605-1

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pGAD424 [520]
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