pCHF30-mHY2 [73]
![pCHF30-mHY2 [73]](images/products/product_info/default_plasmid.png "pCHF30-mHY2 [73]")
Plasmid Information
| Toolbox-No. | 73 |
| Functional Description | phytochromobilin synthase; converts biliverdin to phytochromobilin; this variants mHY2 is without N terminal transit pepdide sequence (is essential for HY2 transport into plastids) |
| Sequence of plasmid | pCHF30-mHY2 show sequence |
| GCK-file (v3.0) | pCHF30-mHY2.gcs |
| GenBank-File | pCHF30-mHY2.gb |
| Key elements of plasmid | pCHF30-mHY2x.pdf |
| Verified via |
Protein Information
| Complete Protein Insert | mHY2-YFP show sequence |
| Protein of Interest | HY2 (N terminal truncated) show sequence |
| Species of Protein | Arabidopsis thaliana |
| Full length or fragment | Fragment |
| Relevant mutations | wt |
| Fusions or Tags - C terminal | YFP |
Vector Information
| Vector name | pCHF30 |
| Type of vector/expressed in | Arabidopsis thaliana |
| Bacterial resistances | spectinomycin |
| High or Low copy | High |
| Growth in standard E. coli | Yes |
| Growth conditions | 37°C |
| Plasmid provided in | DH10B |
Cloning Information
| Insert size (bp) | 865 |
| Cloning Sites 5' - 3' | BglII-XbaI (insert), BamHI- XbaI (vector) |
Additional Information
| Principle Investigator | Prof. Dr. Eberhard Schäfer (⇒ www) |
| Protected Sequences | YFP |
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![pCHF30-mHY2 [73]](images/products/mini/default_plasmid.png "pCHF30-mHY2 [73]")