pIB BFP-dPCNA-Hygro 
NOTE: this plasmid is available for members of the University of Freiburg only!
|Functional Description||Temporal marker:
dPCNA is a temporal marker for S-phase and form many dots during DNA replication that represent replication foci.
|Sequence of plasmid||pIB BFP-dPCNA-Hygro show sequence|
|GCK-file (v3.0)||pIB BFP-dPCNA-Hygro.gcs|
|Key elements of plasmid||pIB BFP-dPCNA-Hygrox.pdf|
digestThis plasmid was verified with restriction digest. Toolbox does not guarantee the correctness of the sequence.
|Complete Protein Insert||BFP-Linker2-dPCNA show sequence|
|Protein of Interest||dPCNA show sequence|
|Species of Protein||Drosohila melanogaster|
|Full length or fragment||Fragment|
|Fusions or Tags - N terminal||BFP, Linker 2|
|Type of vector/expressed in||Drosophila cells|
|Selectable Marker||Blasticidin, Hygromycin|
|High or Low copy||High|
|Growth in standard E. coli||Yes|
|Plasmid provided in||DH10B|
|Insert size (bp)||1554|
|Cloning Sites 5' - 3'||KpnI , HindIII - XbaI, AgeI|
|Principle Investigator||Dr. Patrick Heun (⇒ www)|
|Protected Sequences||Backbone, BFP|
|Special MTA||MTA PCNA|
|Comments||- constitutive Opie2 promoter (strong and slightly weaker that the Actin promoter)
- We had negative experiences with Blasticidin and now mainly co-transfect this vector with hygromycin to obtain stable cell lines.
! BFP derives from Evrogen: www.evrogen.com/products/TagBFP/TagBFP_Notice_to_purchaser.shtml)!
! Linker2-PCNA sequence derives from Dr. Cardoso, TU Darmstadt, is protected with an MTA!The requested scientist has to send the MTA per mail to Prof. Cardoso(email@example.com). Afterwards the signed copy is to send to Toolbox.
|Associated Publications||Distribution of DNA replication proteins in Drosophila cells; Easwaran et al., 2007|
|Vendor Company||Invitrogen (⇒ www)|
|Vendor Order Number||K890-20|