pIB BFP-dPCNA-Hygro [604]
NOTE: this plasmid is available for members of
the University of Freiburg only!
Plasmid Information
Protein Information
Vector Information
| Vector name |
pIB/V5-His |
| Type of vector/expressed in |
Drosophila cells |
| Bacterial resistances |
ampicillin |
| Selectable Marker |
Blasticidin, Hygromycin |
| High or Low copy |
High |
| Growth in standard E. coli |
Yes |
| Growth conditions |
37°C |
| Plasmid provided in |
DH10B |
Cloning Information
| Insert size (bp) |
1554 |
| Cloning Sites 5' - 3' |
KpnI , HindIII - XbaI, AgeI |
Additional Information
| Principle Investigator |
Dr. Patrick Heun (⇒ www) |
| Protected Sequences |
Backbone, BFP |
| Special MTA |
MTA PCNA |
| Comments |
- constitutive Opie2 promoter (strong and slightly weaker that the Actin promoter)
- We had negative experiences with Blasticidin and now mainly co-transfect this vector with hygromycin to obtain stable cell lines.
! BFP derives from Evrogen: www.evrogen.com/products/TagBFP/TagBFP_Notice_to_purchaser.shtml)!
! Linker2-PCNA sequence derives from Dr. Cardoso, TU Darmstadt, is protected with an MTA!The requested scientist has to send the MTA per mail to Prof. Cardoso(cardoso@bio.tu-darmstadt.de). Afterwards the signed copy is to send to Toolbox. |
| Associated Publications |
Distribution of DNA replication proteins in Drosophila cells; Easwaran et al., 2007 |
| Vendor Company |
Invitrogen (⇒ www) |
| Vendor Order Number |
K890-20 |
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