pD h zeta Y0F [56]
Plasmid Information
| Toolbox-No. | 56 |
| Functional Description | subunit of TCR ; plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways |
| Sequence of plasmid | pD h zeta Y0F show sequence |
| GCK-file (v3.0) | pD hz Y0F.gcs |
| GenBank-File | pD hz Y0F.gb |
| Key elements of plasmid | pD hzeta Y0F.pdf |
| Verified via |
Protein Information
| Protein of Interest | h TCR zeta Y0F show sequence |
| Species of Protein | Homo sapiens |
| Full length or fragment | Full Length |
| Relevant mutations | Y64F |
Vector Information
| Vector name | pD alias pRmHA-3 |
| Type of vector/expressed in | Drosophila cells, expressed in S2 cells |
| Bacterial resistances | ampicillin |
| High or Low copy | High |
| Growth in standard E. coli | Yes |
| Growth conditions | 37°C |
| Plasmid provided in | DH10B |
Cloning Information
| Insert size (bp) | 492 |
| Cloning Sites 5' - 3' | EcoRI, XhoI, AvaI - HincII, SalI, XbaI, SalI |
Additional Information
| Principle Investigator | Dr. Wolfgang Schamel (⇒ www) |
| Associated Publications | Analysis of novel phospho-ITAM specific antibodies in a S2 reconstitution system for TCR–CD3 signalling; Dopfer et al., 2010 |
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