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pD h zeta Y0F [56]

pD h zeta Y0F [56]
plasmid map

Plasmid Information

Toolbox-No. 56
Functional Description subunit of TCR ; plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways
Sequence of plasmid pD h zeta Y0F show sequence
GCK-file (v3.0) pD hz Y0F.gcs
GenBank-File pD hz Y0F.gb
Key elements of plasmid pD hzeta Y0F.pdf
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Protein Information

Protein of Interest h TCR zeta Y0F show sequence
Species of Protein Homo sapiens
Full length or fragment Full Length
Relevant mutations Y64F

Vector Information

Vector name pD alias pRmHA-3
Type of vector/expressed in Drosophila cells, expressed in S2 cells
Bacterial resistances ampicillin
High or Low copy High
Growth in standard E. coli Yes
Growth conditions 37°C
Plasmid provided in DH10B

Cloning Information

Insert size (bp) 492
Cloning Sites 5' - 3' EcoRI, XhoI, AvaI - HincII, SalI, XbaI, SalI

Additional Information

Principle Investigator Dr. Wolfgang Schamel (⇒ www)
Associated Publications Analysis of novel phospho-ITAM specific antibodies in a S2 reconstitution system for TCR–CD3 signalling; Dopfer et al., 2010
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