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You are here: Toolbox » Plasmid Shop » pCR8-GW-TOPO_AE5_rhea AE2 [514]

pCR8-GW-TOPO_AE5_rhea AE2 [514]

pCR8-GW-TOPO_AE5_rhea AE2 [514]

NOTE: this plasmid is available for members of the University of Freiburg only!

Plasmid Information

Toolbox-No.	514
Functional Description	Potential BMP-responsive regulatory region of Drosophila rhea AE2 gene in a Gateway(R)vector, contains attL1 and attL2 sites for easy cloning in any Gateway Dest vector
GCK-file (v3.0)	pCR8-GW-TOPO_AE5_rhea AE2.gcc
Verified via	digestThis plasmid was verified with restriction digest. Toolbox does not guarantee the correctness of the sequence. sequencingInsert and flanking restriction site were sequenced by the Toolbox. Detailed information can be found in the GCK-file (see Construct/General Info) and in the GenBank-file (Comment or Info).

Protein Information

Full length or fragment

Fragment

Vector Information

Vector name	pCR8/GW/TOPO
Type of vector/expressed in	bacterias
Bacterial resistances	spectinomycin
High or Low copy	High
Growth in standard E. coli	Yes
Growth conditions	37°C
Plasmid provided in	DH10B

Cloning Information

Insert size (bp)

2767

Additional Information

Principle Investigator	Dr. Giorgos Pyrowolakis (⇒ www)
Protected Sequences	Backbone
Comments	rhea AE2 gene: gb\|AE014296.4\| Drosophila melanogaster chromosome 3L
Vendor Company	Invitrogen (⇒ www)
Vendor Order Number	K252020

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