pDendra2-C [393]
NOTE: this plasmid is available for members of
the University of Freiburg only!
Plasmid Information
| Toolbox-No. |
393 |
| Functional Description |
Vector allows generation of fusions with protein of interest to the C-terminus of Dendra2.
Dendra2 is an improved version of a greentored photoswitchable fluorescent protein Dendra, derived from octocoral Dendronephthya sp. (Gurskaya et al., 2006).(source:Evrogen) |
| Sequence of plasmid |
pDendra2-C
show sequence
|
| GCK-file (v3.0) |
pDendra2-C (Evrogen).gcs |
| GenBank-File |
pDendra2-C (Evrogen).gb |
| Verified via |
|
Protein Information
| Protein of Interest |
Dendra2
show sequence
|
| Species of Protein |
synthetic construct |
| Full length or fragment |
Full Length |
| Relevant mutations |
- |
Vector Information
| Type of vector/expressed in |
mammalian cells |
| Bacterial resistances |
kanamycin |
| Selectable Marker |
Neomycin |
| High or Low copy |
High |
| Growth in standard E. coli |
Yes |
| Growth conditions |
37°C |
| Plasmid provided in |
DH10B |
Cloning Information
| Insert size (bp) |
783 |
| Cloning Sites 5' - 3' |
NheI, SmaI - XbaI, HindIII, SalI, AccI, HincII, SmaI, BamHI, HincII, BsaBI |
Additional Information
| Protected Sequences |
Backbone, Dendra2 |
| Vendor Company |
Evrogen (⇒ www) |
| Vendor Order Number |
FP821 |
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