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You are here: Toolbox » Plasmid Shop » pIB BFP-Cdt [478]

pIB BFP-Cdt [478]

pIB BFP-Cdt [478]

NOTE: this plasmid is available for members of the University of Freiburg only!

plasmid map

Plasmid Information

Toolbox-No.	478
Functional Description	Cell cycle indicator: hCdt1 is the truncated version of the Cdt1 licensing factor for DNA replication. Its only expressed in G1 and low S and described in Sakaue-Sawano, Miyawaki, Cell, 2008. With the appropriate promoters it can be used in other organisms, such as drosophila. BFP-hCdt1 should be coloring G1 cells (low in S-phase) in blue. Awaits verification in Drosophila stable cell lines.
Sequence of plasmid	pIB BFP-Cdt show sequence
PubMed ID	18267078
GCK-file (v3.0)	pIB BFP-Cdt.gcs
GenBank-File	pIB BFP-Cdt.gb
Key elements of plasmid	pIB BFP-Cdtx.pdf
Verified via	digestThis plasmid was verified with restriction digest. Toolbox does not guarantee the correctness of the sequence. sequencingInsert and flanking restriction site were sequenced by the Toolbox. Detailed information can be found in the GCK-file (see Construct/General Info) and in the GenBank-file (Comment or Info).

Protein Information

Complete Protein Insert	BFP-hCdt show sequence
Protein of Interest	hCdt1 show sequence
Species of Protein	Homo sapiens
Full length or fragment	Fragment
Relevant mutations	-
Fusions or Tags - N terminal	BFP (Blue fluorescent protein)

Vector Information

Vector name	pIB/V5-His
Type of vector/expressed in	Drosophila cells
Bacterial resistances	ampicillin
Selectable Marker	Blasticidin
High or Low copy	High
Growth in standard E. coli	Yes
Growth conditions	37°C
Plasmid provided in	DH10B

Cloning Information

Insert size (bp)	1017
Cloning Sites 5' - 3'	ApoI - XbaI, ShoI, SacII, AgeI, ApoI

Additional Information

Principle Investigator	Dr. Patrick Heun (⇒ www)
Protected Sequences	Backbone, BFP
Comments	- constitutive Opie2 promoter (strong and slightly weaker that the Actin promoter) - We had negative experiences with Blasticidin and now mainly co-transfect this vector with hygromycin to obtain stable cell lines (Dr. Patrick Heun).
Vendor Company	Invitrogen (⇒ www)
Vendor Order Number	K890-20

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pIB BFP-Cdt [478]
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