pAcGFP1-Golgi [405]
NOTE: this plasmid is available for members of
the University of Freiburg only!
Plasmid Information
| Toolbox-No. |
405 |
| Functional Description |
Vector is designed for fluorescent labeling of the Golgi apparatus.
AcGFP1 is the green fluorescent protein derives from Aqquorea coerulescens (excitation and emission maxima of AcGFP protein are 575nm and 505nm).(source:Clontech)
The human beta 1,4-galactosyltransferase contains a membrane-anchoring signal peptide that targets the protein to the transmedial region of the Golgi apparatus. |
| Sequence of plasmid |
pAcGFP1-Golgi
show sequence
|
| GCK-file (v3.0) |
pAcGFP1-Golgi(Clontech).gcs |
| GenBank-File |
pAcGFP1-Golgi(Clontech).gb |
| Verified via |
|
Protein Information
| Complete Protein Insert |
pAcGFP1-beta 1,4 GT
show sequence
|
| Protein of Interest |
AcGFP1
show sequence
|
| Species of Protein |
synthetic construct |
| Full length or fragment |
Full Length |
| Relevant mutations |
- |
| Fusions or Tags - N terminal |
N terminal 81 aa of human beta 1,4 GT (galactosyltransferase) |
Vector Information
| Vector name |
pCMV_kan |
| Type of vector/expressed in |
mammalian cells |
| Bacterial resistances |
kanamycin |
| Selectable Marker |
Neomycin |
| High or Low copy |
High |
| Growth in standard E. coli |
Yes |
| Growth conditions |
37°C |
| Plasmid provided in |
DH10B |
Cloning Information
| Insert size (bp) |
981 |
| Cloning Sites 5' - 3' |
NheI - NotI, XbaI |
Additional Information
| Protected Sequences |
EGFP, Backbone |
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