pBPGwZn_AE7_stai AE1 [449]
![pBPGwZn_AE7_stai AE1 [449]](images/products/product_info/default_plasmid.png "pBPGwZn_AE7_stai AE1 [449]")
Plasmid Information
| Toolbox-No. | 449 |
| Functional Description | Potential BMP-responsive regulatory region of Drosophila stai E1 gene fused to lacZ in pBPGwZn. Vector was generate to test the transcriptional activity in transgenic flies. |
| Sequence of plasmid | pBPGwZn_AE7_stai AE1 show sequence |
| PubMed ID | 18621688 |
| GCK-file (v3.0) | pBPGwZn_AE7_stai AE1.gcs |
| GenBank-File | pBPGwZn_AE7_stai AE1.gb |
| Key elements of plasmid | pBPGwZn_AE7_stai AE1x.pdf |
| Verified via |
Protein Information
| Full length or fragment | Fragment |
Vector Information
| Vector name | pBPGwZn |
| Type of vector/expressed in | Drosophila cells; Reporter for generation of transgenic Drosophila via phiC31/attB mediated site specific integration |
| Bacterial resistances | ampicillin |
| High or Low copy | High |
| Growth in standard E. coli | Yes |
| Growth conditions | 37°C |
| Plasmid provided in | DH10B |
Cloning Information
| Insert size (bp) | 2023 |
| Cloning Sites 5' - 3' | EcoRI - EcoRI |
Additional Information
| Principle Investigator | Dr. Giorgos Pyrowolakis (⇒ www) |
| Comments | The regulatory element stai is also available in the GATEWAY vector pc8-GW-TOPO.
stai E1 gene: gb|AE014134.5| Drosophila melanogaster chromosome 2L |
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