pBPGwZn_AE3_ wnt4AE2N [441]
![pBPGwZn_AE3_ wnt4AE2N [441]](images/products/product_info/default_plasmid.png "pBPGwZn_AE3_ wnt4AE2N [441]")
Plasmid Information
| Toolbox-No. | 441 |
| Functional Description | Potential BMP-responsive regulatory region of Drosophila wnt4AE2N gene fused to lacZ in pBPGwZn. To test for transcriptional activity in transgenic flies. |
| Sequence of plasmid | pBPGwZn_AE3_ wnt4AE2N show sequence |
| PubMed ID | 18621688 |
| GCK-file (v3.0) | pBPGwZn_AE3_ wnt4AE2N.gcs |
| GenBank-File | pBPGwZn_AE3_ wnt4AE2N.gb |
| Key elements of plasmid | pBPGwZn_AE3_ wnt4AE2Nx.pdf |
| Verified via |
Protein Information
| Full length or fragment | Fragment |
Vector Information
| Vector name | pBPGwZn |
| Type of vector/expressed in | Drosophila cells; Reporter for generation of transgenic Drosophila via phiC31/attB mediated site specific integration |
| Bacterial resistances | ampicillin |
| High or Low copy | High |
| Growth in standard E. coli | Yes |
| Growth conditions | 37°C |
| Plasmid provided in | DH10B |
Cloning Information
| Insert size (bp) | 2182 |
| Cloning Sites 5' - 3' | EcoRI - EcoRI |
Additional Information
| Principle Investigator | Dr. Giorgos Pyrowolakis (⇒ www) |
| Comments | The insert is also available in the Gateway pc8-GW-TOPO vector.
wnt4AE2N gene: gb|AE014134.5|Drosophila melanogaster chromosome 2L |
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