pD hsp2-CID-GFP-lacI [439]
NOTE: this plasmid is available for members of
the University of Freiburg only!
Plasmid Information
| Toolbox-No. |
439 |
| Functional Description |
This construct allows to visualize centromeres by CID-GFP staining under the inducible hs promoter. The leaky expression is sufficient and non-toxic for this visualization, while higher levels of the centromere specific histone CID have been shown to cause toxicity. The LacI fusion doesn't interfere with this staining and is useful in cells were Lac Operators are integrated into the genome for targeting of the construct to a specific genomic site. |
| Sequence of plasmid |
pD hsp2-CID-GFP-lacI
show sequence
|
| GCK-file (v3.0) |
pD hsp2-CID-GFP-lacI.gcs |
| GenBank-File |
pD hsp2-CID-GFP-lacI.gb |
| Key elements of plasmid |
pD hsp2-CID-GFP-lacIx.pdf |
| Verified via |
|
Protein Information
| Complete Protein Insert |
CID-GFP-lacI
show sequence
|
| Protein of Interest |
CID
show sequence
|
| Species of Protein |
Drosohila melanogaster |
| Full length or fragment |
Full Length |
| Fusions or Tags - C terminal |
EGFP, lacI trunc (lac repressor) |
Vector Information
| Vector name |
pD hsp2 |
| Type of vector/expressed in |
Drosophila cells |
| Bacterial resistances |
ampicillin |
| High or Low copy |
High |
| Growth in standard E. coli |
Yes |
| Growth conditions |
37°C |
| Plasmid provided in |
DH10B |
Cloning Information
| Insert size (bp) |
2553 |
| Cloning Sites 5' - 3' |
SmaI, SpeI - XbaI |
Additional Information
| Principle Investigator |
Dr. Patrick Heun (⇒ www) |
| Protected Sequences |
EGFP |
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