LMP-tomato [409]
| Manufacturer: |
Open Biosystems |
NOTE: this plasmid is available for members of
the University of Freiburg only!
Plasmid Information
| Toolbox-No. |
409 |
| Functional Description |
microRNA-adapted retroviral vector with MSCV derived LTR cassette for expression of shRNAmir constructs of RNA polymerase II. Additional with eukarytotic selectable puromycin marker with PGK promoter and IRES tomato casette . The vector derives from LMP, the original GFP is exchanged with the red fluorescent protein tomato. Further information: http://www.openbiosystems.com/RNAi/RetroviralCloningVectors/ |
| Sequence of plasmid |
LMP-tomato
show sequence
|
| GCK-file (v3.0) |
LMP-tomato(OpenBiosystems).gcs |
| GenBank-File |
LMP(OpenBiosystems).gb |
| Verified via |
|
Protein Information
Vector Information
| Type of vector/expressed in |
Retroviral expression vector for miRNA expression |
| Bacterial resistances |
ampicillin |
| Selectable Marker |
Puromycin |
| High or Low copy |
High |
| Growth in standard E. coli |
Yes |
| Growth conditions |
37°C |
| Plasmid provided in |
DH10B |
Cloning Information
| Cloning Sites 5' - 3' |
XhoI - EcoRI (please notice an additional EcoRI site!) |
Additional Information
| Principle Investigator |
Dr. Hassan Jumaa (⇒ www) |
| Protected Sequences |
Backbone, td tomato |
| Comments |
The vector derives from LMP vector from OpenBiosystems. |
| Vendor Company |
Open Biosystems (⇒ www) |
| Vendor Order Number |
EAV4679 |
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