LMP [408]
| Manufacturer: |
Open Biosystems |
NOTE: this plasmid is available for members of
the University of Freiburg only!
Plasmid Information
| Toolbox-No. |
408 |
| Functional Description |
microRNA-adapted retroviral vector (MSCV/LTRmiR30-PIG) with MSCV derived LTR cassette for expression of shRNAmir constructs of RNA polymerase II. Additional with eukarytotic selectable puromycin marker with PGK promoter and IRES GFP casette. Further information: http://www.openbiosystems.com/RNAi/RetroviralCloningVectors/ |
| Sequence of plasmid |
LMP
show sequence
|
| GCK-file (v3.0) |
LMP(OpenBiosystems).gcs |
| GenBank-File |
LMP(OpenBiosystems).gb |
| Verified via |
|
Protein Information
Vector Information
| Type of vector/expressed in |
Retroviral expression vector for miRNA expression |
| Bacterial resistances |
ampicillin |
| Selectable Marker |
Puromycin |
| High or Low copy |
High |
| Growth in standard E. coli |
Yes |
| Growth conditions |
37°C |
| Plasmid provided in |
DH10B |
Cloning Information
| Cloning Sites 5' - 3' |
XhoI - EcoRI |
Additional Information
| Protected Sequences |
EGFP, Backbone |
| Vendor Company |
Open Biosystems (⇒ www) |
| Vendor Order Number |
EAV4679 |
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