pIB BFP-dGEM trunc [416]
![pIB BFP-dGEM trunc [416]](images/products/product_info/default_plasmid.png "pIB BFP-dGEM trunc [416]")
NOTE: this plasmid is available for members of
the University of Freiburg only!
Plasmid Information
| Toolbox-No. | 416 |
| Functional Description |
|
| Sequence of plasmid | pIB BFP-dGEM trunc show sequence |
| PubMed ID | 18267078; |
| GCK-file (v3.0) | pIB BFP-dGEM trunc.gcs |
| GenBank-File | pIB BFP-dGEM.gb |
| Key elements of plasmid | pIB BFP-dGEMx.pdf |
| Verified via |
Protein Information
| Complete Protein Insert | BFP - dGem trunc show sequence |
| Protein of Interest | dGemini trunc show sequence |
| Species of Protein | Drosohila melanogaster |
| Full length or fragment | Fragment |
| Fusions or Tags - N terminal | BFP |
Vector Information
| Vector name | pIB/V5-His |
| Type of vector/expressed in | Drosophila cells |
| Bacterial resistances | ampicillin |
| Selectable Marker | Blasticidin |
| High or Low copy | High |
| Growth in standard E. coli | Yes |
| Growth conditions | 37°C |
| Plasmid provided in | DH10B |
Cloning Information
| Insert size (bp) | 1125 |
| Cloning Sites 5' - 3' | KpnI - EcoRV, NotI, XhoI, XbaI, SacII |
Additional Information
| Principle Investigator | Dr. Patrick Heun (⇒ www) |
| Protected Sequences | Backbone, BFP |
| Vendor Company | Invitrogen (⇒ www) |
| Vendor Order Number | K890-20 |
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