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You are here: Toolbox » Plasmid Shop » AKAP1(MTS)-linker-mCherry [39]

AKAP1(MTS)-linker-mCherry [39]

AKAP1(MTS)-linker-mCherry [39]

NOTE: this plasmid is available for members of the University of Freiburg only!

plasmid map

Plasmid Information

Toolbox-No.	39
Functional Description	This constructs allows to target mCherry to the mitochondrial outer membrane facing the cytoplasm.
Sequence of plasmid	AKAP1(MTS)-linker-mCherry show sequence
GCK-file (v3.0)	AKAP1(MTS)-linker-mCherry.gcs
GenBank-File	AKAP1(MTS)-linker-mCherry.gb
Key elements of plasmid	AKAP1_MTS_-linker-mCherryx.pdf
Verified via	digestThis plasmid was verified with restriction digest. Toolbox does not guarantee the correctness of the sequence. sequencingInsert and flanking restriction site were sequenced by the Toolbox. Detailed information can be found in the GCK-file (see Construct/General Info) and in the GenBank-file (Comment or Info).

Protein Information

Complete Protein Insert	AKAP1 ( Mitochondrial Targeting Sequence)- mCherry show sequence
Protein of Interest	AKAP1 show sequence
Species of Protein	Mus Musculus
Full length or fragment	Fragment
Relevant mutations	no
Fusions or Tags - C terminal	mCherry

Vector Information

Vector name	pmCherry-N1
Type of vector/expressed in	mammalian cells
Bacterial resistances	Kanamycin
Selectable Marker	Neomycin
High or Low copy	High
Growth in standard E. coli	Yes
Growth conditions	37°C
Plasmid provided in	DH10B

Cloning Information

Insert size (bp)	891bp
Cloning Sites 5' - 3'	XhoI, BglII - NotI, XbaI

Additional Information

Principle Investigator	Dr. Enrico Schmidt (⇒ www)
Protected Sequences	Backbone, mCherry
Vendor Company	Clontech (⇒ www)
Vendor Order Number	632523

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AKAP1(MTS)-linker-mCherry [39]
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