pCMV.LHA-EYFP-µ MHCTM [380]
![pCMV.LHA-EYFP-µ MHCTM [380]](images/products/product_info/default_plasmid.png "pCMV.LHA-EYFP-µ MHCTM [380]")
NOTE: this plasmid is available for members of
the University of Freiburg only!
Plasmid Information
| Toolbox-No. | 380 |
| Functional Description | membrane marker |
| Sequence of plasmid | pCMV.LHA-EYFP-µ MHCTM show sequence |
| GCK-file (v3.0) | pCMV.LHA-EYFP-µ MHCTM.gcs |
| GenBank-File | pCMV.LHA-EYFP-µ MHCTM.gb |
| Key elements of plasmid | pCMV.LHA-EYFP-µ MHCTMx.pdf |
| Verified via |
Protein Information
| Complete Protein Insert | Leader-HA-Tag-YFP-µ heavy chain-MHC TM region show sequence |
| Protein of Interest | mIgM heavy chain CH3+CH4 show sequence |
| Species of Protein | Mus Musculus |
| Full length or fragment | Fragment |
| Relevant mutations | - |
| Fusions or Tags - N terminal | leader sequence of human Ig-alpha precursor; HA-Tag; YFP |
| Fusions or Tags - C terminal | MHCI transmembran region |
Vector Information
| Vector name | pCMV_amp |
| Type of vector/expressed in | mammalian cells |
| Bacterial resistances | ampicillin |
| High or Low copy | High |
| Growth in standard E. coli | Yes |
| Growth conditions | 37°C |
| Plasmid provided in | DH10B |
Cloning Information
| Insert size (bp) | 2079 |
Additional Information
| Principle Investigator | Prof. Dr. Michael Reth (⇒ www) |
| Protected Sequences | Venus |
| Comments | The plasmid gots his name from the PI. Toolbox verified by sequencing YFP not EYFP!! |
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