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pCMV.EGFP-µ MHCTM [379]

pCMV.EGFP-µ MHCTM [379]
NOTE: this plasmid is available for members of the University of Freiburg only!
plasmid map

Plasmid Information

Toolbox-No. 379
Functional Description membrane marker
Sequence of plasmid pCMV.EGFP-µ MHCTM show sequence
GCK-file (v3.0) pCMV EGFP-µ MHCTM.gcs
GenBank-File pCMV.EGFP-µ MHCTM.gb
Key elements of plasmid pCMV.EGFP-µ MHCTMx.pdf
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Protein Information

Complete Protein Insert EGFP-µ heavy chain - MHC TM region show sequence
Protein of Interest mIgM heavy chain CH3+CH4 show sequence
Species of Protein Mus Musculus
Full length or fragment Fragment
Relevant mutations -
Fusions or Tags - N terminal EGFP
Fusions or Tags - C terminal MHC I transmembran region

Vector Information

Vector name pCMV_amp
Type of vector/expressed in mammalian cells
Bacterial resistances ampicillin
High or Low copy High
Growth in standard E. coli Yes
Growth conditions 37°C
Plasmid provided in DH10B

Cloning Information

Insert size (bp) 1929
Cloning Sites 5' - 3' SmaI - ClaI, NotI, EcoRI, EcoRV, XbaI

Additional Information

Principle Investigator Prof. Dr. Michael Reth (⇒ www)
Protected Sequences EGFP
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  1. pCMV.EGFP-µ MHCTM [379]
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