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LMP-MCS puro [131]

LMP-MCS puro [131]
Manufacturer: Open Biosystems
NOTE: this plasmid is available for members of the University of Freiburg only!
plasmid map

Plasmid Information

Toolbox-No. 131
Functional Description only retroviral vector with MCS (Multiple Cloning Site, NotI) and with MSCV derived LTR cassette and eukarytotic selectable puromycin marker with PGK promoter. The 5'miR30 and 3'miR30 sequences and the IRES-GFP casette from the originaly LMP where removed.
GCK-file (v3.0) LMP-MCS puro(OpenBiosystems).gcs
GenBank-File LMP-MCS puro(OpenBiosystems) .gb
Key elements of plasmid LMP-MCS puro(OpenBiosystems) x.pdf
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Vector Information

Type of vector/expressed in Retroviral expression vector with PGK promoter
Bacterial resistances ampicillin
Selectable Marker Puromycin
High or Low copy High
Growth in standard E. coli Yes
Growth conditions 37°C
Plasmid provided in DH10B

Cloning Information

Cloning Sites 5' - 3' -

Additional Information

Principle Investigator Dr. Hassan Jumaa (⇒ www)
Comments Vector is a derivative of the LMP vector (microRNA-adapted retroviral vector; Cat.EAV4679) from Open Biosystems. Please check the website for terms and further information.
Vendor Company Open Biosystems (⇒ www)
Vendor Order Number EAV4679
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