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You are here: Toolbox » Plasmid Shop » pCMV H2B-ECFP.Pac [377]

pCMV H2B-ECFP.Pac [377]

pCMV H2B-ECFP.Pac [377]

NOTE: this plasmid is available for members of the University of Freiburg only!

plasmid map

Plasmid Information

Toolbox-No.	377
Functional Description	Histone H2B is part of the histone octamer that forms the nucleosome in which all eukaryotic genomes are packaged.
Sequence of plasmid	pCMV H2B-ECFP.Pac show sequence
GCK-file (v3.0)	pCMV H2B-ECFP.Pac.gcs
GenBank-File	pCMV H2B-ECFP.Pac.gb
Key elements of plasmid	pCMV H2B-ECFP.Pacx.pdf
Verified via	digestThis plasmid was verified with restriction digest. Toolbox does not guarantee the correctness of the sequence. sequencingInsert and flanking restriction site were sequenced by the Toolbox. Detailed information can be found in the GCK-file (see Construct/General Info) and in the GenBank-file (Comment or Info).

Protein Information

Complete Protein Insert	H2B - ECFP show sequence
Protein of Interest	h H2B show sequence
Species of Protein	Homo sapiens
Full length or fragment	Full Length
Fusions or Tags - C terminal	ECFP

Vector Information

Vector name	pCMV_amp
Type of vector/expressed in	mammalian cells
Bacterial resistances	ampicillin
High or Low copy	High
Growth in standard E. coli	Yes
Growth conditions	37°C
Plasmid provided in	DH10B

Cloning Information

Insert size (bp)	1113
Cloning Sites 5' - 3'	HindIII - NotI, XbaI

Additional Information

Principle Investigator	Prof. Dr. Michael Reth (⇒ www)
Protected Sequences	CFP

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pCMV H2B-ECFP.Pac [377]
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