pGEX-6P1-dPCNA [372]
![pGEX-6P1-dPCNA [372]](images/products/product_info/default_plasmid.png "pGEX-6P1-dPCNA [372]")
Plasmid Information
| Toolbox-No. | 372 |
| Functional Description | Temporal marker:
dPCNA is a temporal marker for S-phase and form many dots during DNA replication that represent replication foci. |
| Sequence of plasmid | pGEX-6P1-dPCNA show sequence |
| GCK-file (v3.0) | pGEX-6P1-dPCNA.gcs |
| GenBank-File | pGEX-6P1-dPCNA.gb |
| Key elements of plasmid | pGEX-6P1-dPCNAx.pdf |
| Verified via |
Protein Information
| Protein of Interest | dPCNA show sequence |
| Species of Protein | Drosohila melanogaster |
| Full length or fragment | Fragment |
Vector Information
| Vector name | pGEX-6P-1 |
| Type of vector/expressed in | bacterial expression vector/ E. coli |
| Bacterial resistances | ampicillin |
| High or Low copy | High |
| Growth in standard E. coli | Yes |
| Growth conditions | 37°C |
| Plasmid provided in | DH10B |
Cloning Information
| Insert size (bp) | 783 |
| Cloning Sites 5' - 3' | BamHI, SmaI, SpeI - Xho NotI |
Additional Information
| Principle Investigator | Dr. Patrick Heun (⇒ www) |
| Protected Sequences | Backbone, PCNA |
| Special MTA | MTA PCNA |
| Comments | The requested scientist has to send the MTA per mail to Prof. Cardoso(cardoso@bio.tu-darmstadt.de). Afterwards the signed copy is to send to Toolbox. |
| Associated Publications | Distribution of DNA replication proteins in Drosophila cells; Easwaran et al., 2007 |
| Vendor Company | GE Healthcare (⇒ www) |
| Vendor Order Number | 27-4597 |
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