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pD GFPSyk LK [361]

pD GFPSyk LK [361]
NOTE: this plasmid is available for members of the University of Freiburg only!
plasmid map

Plasmid Information

Toolbox-No. 361
Functional Description


Syk mutant expressing a linker and the catalytic domain in fusion with N-terminal GFP


Sequence of plasmid pD GFPSyk LK show sequence
PubMed ID 12453414
GCK-file (v3.0) pD GFPSyk LK.gcs
GenBank-File pD-GFPSyk LK.gb
Key elements of plasmid pD GFPSyk LKx.pdf
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Protein Information

Complete Protein Insert GFP - Syk LK show sequence
Protein of Interest h Syk LK show sequence
Species of Protein Homo sapiens
Full length or fragment Fragment
Relevant mutations only catalytic domain of Syk
Fusions or Tags - N terminal GFP, LK= Linker

Vector Information

Vector name pD alias pRmHA-3
Type of vector/expressed in Drosophila cells, expressed in S2 cells
Bacterial resistances ampicilllin
High or Low copy High
Growth in standard E. coli Yes
Growth conditions 37°C
Plasmid provided in DH10B

Cloning Information

Insert size (bp) 1809
Cloning Sites 5' - 3' EcoRI - SalI

Additional Information

Principle Investigator Prof. Dr. Michael Reth (⇒ www)
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