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You are here: Toolbox » Plasmid Shop » pD-hSykdSH2N [360]

pD-hSykdSH2N [360]

pD-hSykdSH2N [360]

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Plasmid Information

Toolbox-No.	360
Functional Description	Syk mutant with deleted N-terminal SH2 domain
Sequence of plasmid	pD-hSykdSH2N show sequence
PubMed ID	12453414
GCK-file (v3.0)	pD-hSykdSH2N.gcs
GenBank-File	pD-hSykdSH2N.gb
Key elements of plasmid	pD hSykdSH2Nx.pdf
Verified via	digestThis plasmid was verified with restriction digest. Toolbox does not guarantee the correctness of the sequence. sequencingInsert and flanking restriction site were sequenced by the Toolbox. Detailed information can be found in the GCK-file (see Construct/General Info) and in the GenBank-file (Comment or Info).

Protein Information

Protein of Interest	h Syk dSH2N show sequence
Species of Protein	Homo sapiens
Full length or fragment	Fragment
Relevant mutations	N-terminal SH2 domain deleted: aa110-635

Vector Information

Vector name	pD alias pRmHA-3
Type of vector/expressed in	Drosophila cells, expressed in S2 cells
Bacterial resistances	ampicilllin
High or Low copy	High
Growth in standard E. coli	Yes
Growth conditions	37°C
Plasmid provided in	DH10B

Cloning Information

Insert size (bp)	1596
Cloning Sites 5' - 3'	EcoRI - EcoRI

Additional Information

Principle Investigator

Prof. Dr. Michael Reth (⇒ www)

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pD-hSykdSH2N [360]
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