pMOWS-NtmNLNN-GFP [320]
![pMOWS-NtmNLNN-GFP [320]](images/products/product_info/default_plasmid.png "pMOWS-NtmNLNN-GFP [320]")
NOTE: this plasmid is available for members of
the University of Freiburg only!
Plasmid Information
| Toolbox-No. | 320 |
| Functional Description | LAT/NTAL swapping mutant; L=LAT, N=NTAL |
| Sequence of plasmid | pMOWS-NtmNLNN-GFP show sequence |
| GCK-file (v3.0) | pMOWS-NtmNLNN-GFP.gcs |
| GenBank-File | pMOWS-NtmNLNN-GFP.gb |
| Key elements of plasmid | pMOWS-NtmNLNN-GFPx.pdf |
| Verified via |
Protein Information
| Complete Protein Insert | LAT 2 (Ntm) - LAT2 (N1) - LAT(L1) - LAT2 (N2,3) - GFP show sequence |
| Full length or fragment | Fragment |
| Relevant mutations | no |
| Fusions or Tags - C terminal | GFP |
Vector Information
| Vector name | pMOWS |
| Type of vector/expressed in | mammalian cells, hematopoietic cell lines and progenitor cells |
| Bacterial resistances | ampicillin |
| Selectable Marker | Puromycin |
| High or Low copy | High |
| Growth in standard E. coli | Yes |
| Growth conditions | 37°C |
| Plasmid provided in | XL-1 Blue |
Cloning Information
| Insert size (bp) | 1389 |
| Cloning Sites 5' - 3' | PacI - (EcoRI-site also in L1!, BamHI- without STOP) |
Additional Information
| Principle Investigator | Dr. Hassan Jumaa (⇒ www) |
| Protected Sequences | EGFP |
| Associated Publications | The N Terminus of the Non-T Cell Activation Linker (NTAL) Confers Inhibitory Effects on Pre-B Cell Differentiation1; Herzog, Jumaa, The Journal of Imm |
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