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pMOWS-NtmNLL-GFP [184]

pMOWS-NtmNLL-GFP [184]
NOTE: this plasmid is available for members of the University of Freiburg only!
plasmid map

Plasmid Information

Toolbox-No. 184
Functional Description LAT/NTAL swapping mutant; fragment1: exon1 (TM-region), fragment2: exons 2-6, fragment3: exons 7-8, fragment4: exons 9-11; L=LAT, N=NTAL
Sequence of plasmid pMOWS-NtmNLL-GFP show sequence
GCK-file (v3.0) pMOWS-NtmNLL-GFP.gcs
GenBank-File pMOWS-NtmNLL-GFP.gb
Key elements of plasmid pMOWS-NtmNLL-GFPx.pdf
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Protein Information

Complete Protein Insert Ntm - N - L - L - GFP show sequence
Protein of Interest m N1tm N1 L2 L3 show sequence
Species of Protein Mus Musculus
Full length or fragment Fragment
Relevant mutations no
Fusions or Tags - C terminal GFP

Vector Information

Vector name pMOWS
Type of vector/expressed in mammalian cells, hematopoietic cell lines and progenitor cells
Bacterial resistances ampicillin
Selectable Marker Puromycin
High or Low copy High
Growth in standard E. coli Yes
Growth conditions 37°C
Plasmid provided in XL1-Blue

Cloning Information

Insert size (bp) 1440
Cloning Sites 5' - 3' PacI - EcoRI

Additional Information

Principle Investigator Dr. Hassan Jumaa (⇒ www)
Protected Sequences EGFP
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