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You are here: Toolbox » Plasmid Shop » pMOWS-N1Nmut2L3-GFP [174]

pMOWS-N1Nmut2L3-GFP [174]

pMOWS-N1Nmut2L3-GFP [174]

NOTE: this plasmid is available for members of the University of Freiburg only!

plasmid map

Plasmid Information

Toolbox-No.	174
Functional Description	LAT/NTAL swapping mutant; fragment1: exons 1-6, fragment2: exons 7-8, fragment3: exons 9-11; L=LAT, N=NTAL
Sequence of plasmid	pMOWS-N1Nmut2L3-GFP show sequence
GCK-file (v3.0)	pMOWS-N1Nmut2L3-GFP.gcs
GenBank-File	plasmid_pMOWS-N1Nmut2L3-GFP.gb
Key elements of plasmid	pMOWS-N1Nmut2L3-GFPx.pdf
Verified via	digestThis plasmid was verified with restriction digest. Toolbox does not guarantee the correctness of the sequence. sequencingInsert and flanking restriction site were sequenced by the Toolbox. Detailed information can be found in the GCK-file (see Construct/General Info) and in the GenBank-file (Comment or Info).

Protein Information

Complete Protein Insert	N1 Nmut2 (LAT2) L3 (LAT) - GFP show sequence
Protein of Interest	m N1 N2mut L3 show sequence
Species of Protein	Mus Musculus
Full length or fragment	Fragment
Relevant mutations	aa 117-120: V D P I -> L V V L
Fusions or Tags - C terminal	GFP

Vector Information

Vector name	pMOWS
Type of vector/expressed in	mammalian cells, hematopoietic cell lines and progenitor cells
Bacterial resistances	ampicillin
Selectable Marker	Puromycin
High or Low copy	High
Growth in standard E. coli	Yes
Growth conditions	37°C
Plasmid provided in	DH10B

Cloning Information

Insert size (bp)	1356
Cloning Sites 5' - 3'	PacI - EcoRI

Additional Information

Principle Investigator	Dr. Hassan Jumaa (⇒ www)
Protected Sequences	EGFP

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pMOWS-N1Nmut2L3-GFP [174]
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