LOCUS Pex14_S310DS313A-HTB 7735 bp DNA circular COMMENT Toolbox sequenced area from position 3366 to 4425. DEFINITION Toolbox sequenced area from position 3366 to 4425. FEATURES Location/Qualifiers misc_feature complement(65..583) /label=Cen6_ARS4 CDS 715..1575 /label=ampR misc_feature 1730..2349 /label=pBR322ori CDS 3452..4840 /label= Pex14_S310DS313A-HTB misc_feature 3452..4474 /label= Pex14_S310DS313A /note="NP_011362.1 Pex14p [Saccharomyces cerevisiae S288c] sp|P53112.1|PEX14_Peroxisomal membrane protein PEX14; AltName: Full=Peroxin-14; complete protein; relevant mutation S310D, S313A" misc_feature 4505..4522 /label=6xHis misc_feature 4505..4840 /label= HTB-Tag misc_feature 4864..5043 /label= yADH1 misc_feature 5235..5378 /label=TRP1(0-143) misc_feature complement(6519..7319) /label=URA3 ORIGIN 1 GACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGGACGGATCGCTTGCCTGTAACTTACACGCGCCTCGTATCTTTTAATGATGGAATAATTTGGGAATTTACTCTGTGTTTATTTA 149 TTTTTATGTTTTGTATTTGGATTTTAGAAAGTAAATAAAGAAGGTAGAAGAGTTACGGAATGAAGAAAAAAAAATAAACAAAGGTTTAAAAAATTTCAACAAAAAGCGTACTTTACATATATATTTATTAGACAAGAAAAGCAGATTA 297 AATAGATATACATTCGATTAACGATAAGTAAAATGTAAAATCACAGGATTTTCGTGTGTGGTCTTCTACACAGACAAGATGAAACAATTCGGCATTAATACCTGAGAGCAGGAAGAGCAAGATAAAAGGTAGTATTTGTTGGCGATCC 445 CCCTAGAGTCTTTTACATCTTCGGAAAACAAAAACTATTTTTTCTTTAATTTCTTTTTTTACTTTCTATTTTTAATTTATATATTTATATTAAAAAATTTAAATTATAATTATTTTTATAGCACGTGATGAAAAGGACCCAGGTGGCA 593 CTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT ATG AGT ATT CAA CAT TTC 733 CGT GTC GCC CTT ATT CCC TTT TTT GCG GCA TTT TGC CTT CCT GTT TTT GCT CAC CCA GAA ACG CTG GTG AAA GTA AAA GAT GCT GAA GAT CAG TTG GGT GCA CGA GTG GGT 844 TAC ATC GAA CTG GAT CTC AAC AGC GGT AAG ATC CTT GAG AGT TTT CGC CCC GAA GAA CGT TTT CCA ATG ATG AGC ACT TTT AAA GTT CTG CTA TGT GGC GCG GTA TTA TCC 955 CGT ATT GAC GCC GGG CAA GAG CAA CTC GGT CGC CGC ATA CAC TAT TCT CAG AAT GAC TTG GTT GAG TAC TCA CCA GTC ACA GAA AAG CAT CTT ACG GAT GGC ATG ACA GTA 1066 AGA GAA TTA TGC AGT GCT GCC ATA ACC ATG AGT GAT AAC ACT GCG GCC AAC TTA CTT CTG ACA ACG ATC GGA GGA CCG AAG GAG CTA ACC GCT TTT TTG CAC AAC ATG GGG 1177 GAT CAT GTA ACT CGC CTT GAT CGT TGG GAA CCG GAG CTG AAT GAA GCC ATA CCA AAC GAC GAG CGT GAC ACC ACG ATG CCT GTA GCA ATG GCA ACA ACG TTG CGC AAA CTA 1288 TTA ACT GGC GAA CTA CTT ACT CTA GCT TCC CGG CAA CAA TTA ATA GAC TGG ATG GAG GCG GAT AAA GTT GCA GGA CCA CTT CTG CGC TCG GCC CTT CCG GCT GGC TGG TTT 1399 ATT GCT GAT AAA TCT GGA GCC GGT GAG CGT GGG TCT CGC GGT ATC ATT GCA GCA CTG GGG CCA GAT GGT AAG CCC TCC CGT ATC GTA GTT ATC TAC ACG ACG GGG AGT CAG 1510 GCA ACT ATG GAT GAA CGA AAT AGA CAG ATC GCT GAG ATA GGT GCC TCA CTG ATT AAG CAT TGG TAA CTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTT 1636 AAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGC 1784 AAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAA 1932 CTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGC 2080 ACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGC 2228 TTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTG 2376 CTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCA 2524 ATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTACCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGC 2672 TTCCGGCTCCTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCGCGCAATTAACCCTCACTAAAGGGAACAAAAGCTGGAGCTCCACCGCGGTGGCGGCCGCTCTAGAAC 2820 TAGTGGATCCACTAGTAGGCCTCCCGCCATAATTGCACGGAGAAGCGATATCAATTCCGACATCCTGTATTCATCTGTACTTACACATTTTACGAGGAACATAGCTACATATTGTTCACCGATGGTCATGCTAAATGGAAGAAAACGA 2968 TTGTTGTCTAAGAATGGCTTACCGAAGCTGCCCTTGTCAAATTTCAGCTCTTGAAAATTTAAGCCCGTTACTATAGAGCAGCCAAACAACTGCAGCAGCTGGCTGCATAGATTTGAACATCTATCGTGAAACGATTTTTTATTGAGGA 3116 TTCTGGCTTGAGACGCCAATGGCAAAGTTCTCATTAATGCCTCGAACGTAAACTCATCCGCGAGTATATCCTCTTGAATTTCAACAACGAATATACCTGCCCATGGTCTTACACCTGCCACCTTTGAAACTTCGCTTACTACTTCAGT 3264 CGTTTTAACCATCCACGGTTTTTTTGCTGAGTGATTCTCTTTCTCCTCATTCTCATTTTAGTCATAGCGGTTTTAATAAGCGCCCGAAAGATAATTGTAAAACATATATTCAATGCTTAAAAATATAAGAAATTGCCCATCAATTTGA 3412 AAACTCAAGTAAAACAGAGAAGTTGTAAGGTGAATAAGGA ATG AGT GAC GTG GTC AGT AAA GAT CGT AAG GCA TTG TTC GAC TCA GCA GTA TCC TTT TTA AAG GAT GAG TCC ATT AAA GAT 3533 GCT CCA CTT TTA AAG AAA ATC GAA TTT TTA AAA TCC AAA GGG TTA ACA GAA AAG GAG ATT GAA ATA GCC ATG AAA GAG CCC AAG AAA GAC GGT ATC GTA GGC GAT GAA GTA 3644 TCG AAA AAA ATT GGT AGT ACT GAG AAT AGA GCC TCA CAG GAT ATG TAT CTC TAT GAA GCG ATG CCA CCA ACG CTG CCC CAC AGG GAT TGG AAG GAC TAT TTT GTG ATG GCT 3755 ACT GCC ACA GCT GGG CTG TTG TAT GGT GCA TAT GAA GTA ACT AGA AGG TAT GTG ATA CCA AAT ATT TTA CCA GAA GCA AAA AGC AAG TTG GAA GGG GAC AAA AAA GAA ATT 3866 GAT GAT CAG TTC TCC AAA ATC GAT ACA GTC CTC AAT GCC ATC GAA GCG GAG CAA GCT GAG TTT AGG AAA AAG GAA AGC GAA ACA TTA AAG GAA CTT AGT GAC ACG ATT GCT 3977 GAA CTG AAA CAG GCG CTT GTG CAG ACA ACA AGA AGC AGG GAA AAG ATC GAA GAC GAG TTT AGA ATA GTT AAA CTC GAG GTG GTC AAT ATG CAA AAT ACG ATC GAC AAA TTT 4088 GTT TCA GAT AAT GAC GGC ATG CAA GAG TTA AAT AAT ATC CAA AAA GAA ATG GAA TCT CTG AAA AGC TTA ATG AAT AAC CGT ATG GAA TCC GGT AAT GCG CAG GAC AAC AGA 4199 TTA TTT TCC ATA TCT CCT AAT GGT ATA CCT GGC ATA GAT ACG ATT CCA TCT GCG TCT GAG ATT CTT GCC AAA ATG GGC ATG CAA GAA GAA AGT GAT AAA GAA AAG GAA AAC 4310 GGC AGC GAT GCT AAT AAA GAT GAC AAT GCT GTT CCA GCG TGG AAA AAA GCA AGA GAA CAA ACT ATT GAT GAT AAC GCC GCT ATT CCA GAA TGG CAA AAA AAT ACC GCC GCC 4421 AAT GAG ATC AGT GTC CCT GAC TGG CAA AAT GGA CAG GTC GAA GAC TCC ATC CCA CGG ATC CCC GGG TTA ATT AAC AGG GGT TCA CAT CAT CAC CAC CAT CAT GAC TAC GAT 4532 ATA CCC ACA ACC GCC AGC GAG AAT CTG TAC TTT CAA TCA GAG TTA AAA ACC GCC GCA CTA GCT CAA CAT GAC GAG GCT GCT GGA AAG GCC GGT GAA GGT GAA ATC CCT GCC 4643 CCT CTT GCT GGT ACC GTT TCT AAG ATA CTG GTA AAA GAA GGT GAC ACT GTT AAA GCT GGT CAA ACA GTT CTG GTG CTG GAG GCT ATG AAA ATG GAG ACA GAA ATT AAC GCT 4754 CCT ACT GAC GGA AAA GTT GAA AAG GTG TTA GTT AAG GAA AGA GAT GCT GTT CAA GGT GGT CAA GGT CTA ATC AAG ATC GGC GTT TAA TGAGGCGCGCCACTTCTAAATAAGCGAATTTC 4873 TTATGATTTATGATTTTTATTATTAAATAAGTTATAAAAAAAATAAGTGTATACAAATTTTAAAGTGACTCTTAGGTTTTAAAACGAAAATTCTTATTCTTGAGTAACTCTTTCCTGTAGGTCAGGTTGCTTTCTCAGGTATAGTATG 5021 AGGTCGCTCTTATTGACCACACCTCTACCGGCAGATCCGCTAGGGATAACAGGGTAATATAGATCTGTACAATCTTGATCCGGAGCTTTTCTTTTTTTGCCGATTAAGAATTAATTCGGTCGAAAAAAGAAAAGGAGAGGGCCAAGAG 5169 GGAGGGCATTGGTGACTATTGAGCACGTGAGTATACGTGATTAAGCACACAAAGGCAGCTTGGAGTATGTCTGTTATTAATTTCACAGGTAGTTCTGGTCCATTGGTGAAAGTTTGCGGCTTGCAGAGCACAGAGGCCGCAGAATGTG 5317 CTCTAGATTCCGATGCTGACTTGCTGGGTATTATATGTGTGCCCAATAGAAAGAGAACAATTGACCTCCATGGAATTTTCGAACCTTTACAAGGAATTCTTTAATGAAAAGTTCCCGTCTTTGATTCAAGAGGAAGAAGAAGATACAA 5465 CAACAACTGCAAATATAAATGAGGTCAAGAAAGCGTCCGATTTGGTGGATACACCCAGCAACAACACGGCTGCTACTGCAGATACTACACACTTACACGAGGCTCTAGATATTGTTTGCTCCGATTTTGTTAAAATATTAAACCTGGA 5613 GAAGCCTCTTATCTTGGCAGATTATATCGTTGAGGTATTGCTTGTGAACTACAACTCAGATATGATAAAATGCTTTCTGCCTAAATTGAATTCCGTTAGGAACTCCTTGCTATTGGTACCCAATTCGCCCTATAGTGAGTCGTATTAC 5761 GCGCGCTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCA 5909 GCCTGAATGGCGAATGGCGCGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGC 6057 CGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACG 6205 TTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACG 6353 CGAATTTTAACAAAATATTAACGTTTACAATTTCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATAGGGTAATAACTGATATAATTAAATTGAAGCTCTAATTTGTGAGTTTAGTATACATGCATTTAC 6501 TTATAATACAGTTTTTTAGTTTTGCTGGCCGCATCTTCTCAAATATGCTTCCCAGCCTGCTTTTCTGTAACGTTCACCCTCTACCTTAGCATCCCTTCCCTTTGCAAATAGTCCTCTTCCAACAATAATAATGTCAGATCCTGTAGAG 6649 ACCACATCATCCACGGTTCTATACTGTTGACCCAATGCGTCTCCCTTGTCATCTAAACCCACACCGGGTGTCATAATCAACCAATCGTAACCTTCATCTCTTCCACCCATGTCTCTTTGAGCAATAAAGCCGATAACAAAATCTTTGT 6797 CGCTCTTCGCAATGTCAACAGTACCCTTAGTATATTCTCCAGTAGATAGGGAGCCCTTGCATGACAATTCTGCTAACATCAAAAGGCCTCTAGGTTCCTTTGTTACTTCTTCTGCCGCCTGCTTCAAACCGCTAACAATACCTGGGCC 6945 CACCACACCGTGTGCATTCGTAATGTCTGCCCATTCTGCTATTCTGTATACACCCGCAGAGTACTGCAATTTGACTGTATTACCAATGTCAGCAAATTTTCTGTCTTCGAAGAGTAAAAAATTGTACTTGGCGGATAATGCCTTTAGC 7093 GGCTTAACTGTGCCCTCCATGGAAAAATCAGTCAAGATATCCACATGTGTTTTTAGTAAACAAATTTTGGGACCTAATGCTTCAACTAACTCCAGTAATTCCTTGGTGGTACGAACATCCAATGAAGCACACAAGTTTGTTTGCTTTT 7241 CGTGCATGATATTAAATAGCTTGGCAGCAACAGGACTAGGATGAGTAGCAGCACGTTCCTTATATGTAGCTTTCGACATGATTTATCTTCGTTTCCTGCAGGTTTTTGTTCTGTGCAGTTGGGTTAAGAATACTGGGCAATTTCATGT 7389 TTCTTCAACACTACATATGCGTATATATACCAATCTAAGTCTGTGCTCCTTCCTTCGTTCTTCCTTCTGTTCGGAGATTACCGAATCAAAAAAATTTCAAAGAAACCGAAATCAAAAAAAAGAATAAAAAAAAAATGATGAATTGAAT 7537 TGAAAAGCTGTGGTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCT 7685 CCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGA //