r84GACGGATCGGGAGATCTCCCGATCCCCTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTGCTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACCCACTGCTTACTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTGGCTAGCGTTTAAACTTAAGCTTGGTACCGAGCTCGGATCCACTAGTCCAGTGTGGTGGAATTCTGCAGATATCCAGCACAGTGGCGGCCGCTCGAGTCTAGAGGGCCCGTTTAAACCCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGCTTCTGAGGCGGAAAGAACCAGCTGGGGCTCTAGGGGGTATCCCCACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTAATTCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGTATATCCATTTTCGGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCTGCCATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCACCCCAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGTATACCGTCGACCTCTAGCTAGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTC4R kR k4 kT4 4    3   RR6   S RRPv   SW r   SDS6e    SR)    S`v R    ^q    $l  CMV_IECMV immearrly promoterBGH pABGH polyadenylation sitef1 oriNeoRNeomycin resistance geneSV40 pApUC oriampRT74 ~D~ a` )U! ()n$H  \ NX>! ()n $  x jc> () n-$   m?C ()-nQ$   t?L9 ()Qnu$   y?zy ()un$   {? ()n$H  {? ()nD  x?} ()nNheIIsoschizomer with AceII, AsuNHI, BmtI, BspOI, LlaG2I, and PstNHI. Available from Invitrogen Corporation (8/08), Minotech Biotechnology (8/08), Fermentas International Inc. (8/08), Nippon Gene Co., Ltd. (8/08), Takara Bio Inc. (8/08), Roche Applied Science (8/08), New England Biolabs (8/08), Toyobo Biochemicals (8/08), Promega Corporation (8/08), Sigma Chemical Corporation (8/08), Bangalore Genei (8/08), and MP Biomedicals (8/08). PmeI_Isoschizomer with MssI. Available from New England Biolabs (8/08), and MP Biomedicals (8/08). HindIIIhIsoschizomer with Asp52I, Asp3065I, BbrI, BbrAI, BpeI, BseHI, BsmGII, BspKT8I, BspLAIII, Bst170II, BstFI, BstZ1II, Cfr32I, ChuI, Csp231I, Eco65I, Eco98I, Eco188I, Eco231I, EcoVIII, Hin5III, Hin173I, Hin1076III, HinJCII, HinSAFI, HinbIII, HinfII, HsuI, LlaCI, MkiI, SpaPIV, SsbI, Uba83I, Uba1164II, Uba1219I, and Uba1435I. Available from Invitrogen Corporation (8/08), Minotech Biotechnology (8/08), Fermentas International Inc. (8/08), American Allied Biochemical, Inc. (8/08), SibEnzyme Ltd. (8/08), Nippon Gene Co., Ltd. (8/08), Takara Bio Inc. (8/08), Roche Applied Science (8/08), New England Biolabs (8/08), Toyobo Biochemicals (8/08), Molecular Biology Resources - CHIMERx (8/08), Promega Corporation (8/08), Sigma Chemical Corporation (8/08), Bangalore Genei (8/08), Vivantis Technologies (8/08), MP Biomedicals (8/08), EURx Ltd. (8/08), and CinnaGen Inc. (8/08). KpnI;Isoschizomer with Acc65I, AhaB8I, Asp718I, BspJ106I, Eco149I, Esp19I, KpnK14I, MvsI, MvsAI, MvsBI, MvsCI, MvsDI, MvsEI, NmiI, Sau10I, SthI, SthAI, SthBI, SthCI, SthDI, SthEI, SthFI, SthGI, SthHI, SthJI, SthKI, SthLI, SthMI, SthNI, Uba76I, Uba85I, Uba86I, Uba87I, and Uba1201I. Available from Invitrogen Corporation (8/08), Minotech Biotechnology (8/08), Fermentas International Inc. (8/08), American Allied Biochemical, Inc. (8/08), SibEnzyme Ltd. (8/08), Nippon Gene Co., Ltd. (8/08), Takara Bio Inc. (8/08), Roche Applied Science (8/08), New England Biolabs (8/08), Toyobo Biochemicals (8/08), Molecular Biology Resources - CHIMERx (8/08), Promega Corporation (8/08), Sigma Chemical Corporation (8/08), Bangalore Genei (8/08), Vivantis Technologies (8/08), MP Biomedicals (8/08), EURx Ltd. (8/08), and CinnaGen Inc. (8/08). BamHI5Isoschizomer with AacI, AaeI, AcaII, AccEBI, AinII, AliI, Ali12257I, Ali12258I, ApaCI, AsiI, AspTII, Atu1II, BamFI, BamKI, BamNI, Bca1259I, Bce751I, Bco10278I, BnaI, BsaDI, Bsp30I, Bsp46I, Bsp90II, Bsp98I, Bsp130I, Bsp131I, Bsp144I, Bsp4009I, BspAAIII, BstI, Bst1126I, Bst2464I, Bst2902I, BstQI, Bsu90I, Bsu8565I, Bsu8646I, BsuB519I, BsuB763I, CelI, DdsI, GdoI, GinI, GoxI, GseIII, GstI, MleI, Mlu23I, NasBI, Nsp29132II, NspSAIV, OkrAI, Pac1110I, Pae177I, Pfl8I, Psp56I, RhsI, Rlu4I, RspLKII, SolI, SpvI, SurI, Uba19I, Uba31I, Uba38I, Uba51I, Uba88I, Uba1098I, Uba1163I, Uba1167I, Uba1172I, Uba1173I, Uba1205I, Uba1224I, Uba1242I, Uba1250I, Uba1258I, Uba1297I, Uba1302I, Uba1324I, Uba1325I, Uba1334I, Uba1339I, Uba1346I, Uba1383I, Uba1398I, Uba1402I, Uba1414I, Uba4009I, and UbaN3I. Available from Invitrogen Corporation (8/08), Minotech Biotechnology (8/08), Fermentas International Inc. (8/08), American Allied Biochemical, Inc. (8/08), SibEnzyme Ltd. (8/08), Nippon Gene Co., Ltd. (8/08), Takara Bio Inc. (8/08), Roche Applied Science (8/08), New England Biolabs (8/08), Toyobo Biochemicals (8/08), Molecular Biology Resources - CHIMERx (8/08), Promega Corporation (8/08), Sigma Chemical Corporation (8/08), Bangalore Genei (8/08), Vivantis Technologies (8/08), MP Biomedicals (8/08), EURx Ltd. (8/08), and CinnaGen Inc. (8/08). EcoRIIsoschizomer with Eco82I, Eco159I, Eco228I, Eco237I, Eco252I, FunII, HalI, Hal22I, Kpn49kI, Ppu111I, RsrI, Srl32DII, Srl55DI, SsoI, Uba58I, Van91II, VchN100I, and VchO2I. Available from Invitrogen Corporation (8/08), Minotech Biotechnology (8/08), Fermentas International Inc. (8/08), American Allied Biochemical, Inc. (8/08), SibEnzyme Ltd. (8/08), Nippon Gene Co., Ltd. (8/08), Takara Bio Inc. (8/08), Roche Applied Science (8/08), New England Biolabs (8/08), Toyobo Biochemicals (8/08), Molecular Biology Resources - CHIMERx (8/08), Promega Corporation (8/08), Sigma Chemical Corporation (8/08), Bangalore Genei (8/08), Vivantis Technologies (8/08), MP Biomedicals (8/08), EURx Ltd. (8/08), and CinnaGen Inc. (8/08). EcoRVIsoschizomer with Bsc217I, BshLI, BsoAI, Bsp16I, BstRI, CeqI, Eco17I, Eco32I, Eco178I, HjaI, HpyCI, NanI, NflAI, NsiCI, Pac1110II, Pfl16I, PxyARI, Rma495II, Rma496II, Rma497II, SmiMII, Tsp273I, Uba1400I, and UbaN4I. Available from Invitrogen Corporation (8/08), Minotech Biotechnology (8/08), American Allied Biochemical, Inc. (8/08), SibEnzyme Ltd. (8/08), Nippon Gene Co., Ltd. (8/08), Takara Bio Inc. (8/08), Roche Applied Science (8/08), New England Biolabs (8/08), Toyobo Biochemicals (8/08), Molecular Biology Resources - CHIMERx (8/08), Promega Corporation (8/08), Sigma Chemical Corporation (8/08), Bangalore Genei (8/08), Vivantis Technologies (8/08), MP Biomedicals (8/08), EURx Ltd. (8/08), and CinnaGen Inc. (8/08). NotI#Isoschizomer with CciNI, CsiBI, CspBI, and MchAI. Available from Invitrogen Corporation (8/08), Minotech Biotechnology (8/08), Fermentas International Inc. (8/08), American Allied Biochemical, Inc. (8/08), Nippon Gene Co., Ltd. (8/08), Takara Bio Inc. (8/08), Roche Applied Science (8/08), New England Biolabs (8/08), Toyobo Biochemicals (8/08), Molecular Biology Resources - CHIMERx (8/08), Promega Corporation (8/08), Sigma Chemical Corporation (8/08), Bangalore Genei (8/08), MP Biomedicals (8/08), EURx Ltd. (8/08), and CinnaGen Inc. (8/08). XhoIIsoschizomer with AbrI, AerAI, AhyAI, AlwFII, Asp15I, Asp47I, Asp703I, BadI, BbfI, BbiIII, BluI, Bsp92I, Bsp129I, Bsp139I, Bsp140I, Bsp141I, Bsp142I, BspAAI, BssHI, BstHI, BstLI, BstVI, BsuMI, BthI, CcrI, CjaI, DdeII, DrdDI, Eae2I, EscI, MavI, McaI, MecI, MhaI, MlaAI, MpuI, MrhI, MscAI, MsiI, Msp23II, OcoI, PaeR7I, PanI, Pfl67I, PflNI, PflWI, Psp4I, PspNI, Sal1974I, Sau3239I, SauLPII, Sbi68I, Sca1827I, SciI, Sci1831I, SciBI, ScuI, SdiAI, SexI, Sfr274I, Sfu1762I, SgaI, SgoI, Sgr1841I, Shy1766I, SlaI, SluI, Sol10179I, SpaI, Sph1719I, SrifpI, Ssp4I, StaAI, StrI, Sve194I, TliI, Uba1130I, Uba1148I, Uba1154I, Uba1166I, Uba1237I, Uba1248I, Uba1271I, Uba1298I, Uba1335I, Uba1397I, Uba1448I, and XpaI. Available from Invitrogen Corporation (8/08), Fermentas International Inc. (8/08), American Allied Biochemical, Inc. (8/08), Nippon Gene Co., Ltd. (8/08), Takara Bio Inc. (8/08), Roche Applied Science (8/08), New England Biolabs (8/08), Toyobo Biochemicals (8/08), Molecular Biology Resources - CHIMERx (8/08), Promega Corporation (8/08), Sigma Chemical Corporation (8/08), Bangalore Genei (8/08), MP Biomedicals (8/08), EURx Ltd. (8/08), and CinnaGen Inc. (8/08). XbaI_Isoschizomer with BspAAII, BspLU11II, BsrXI, and Msp23I. Available from Invitrogen Corporation (8/08), Minotech Biotechnology (8/08), Fermentas International Inc. (8/08), American Allied Biochemical, Inc. (8/08), SibEnzyme Ltd. (8/08), Nippon Gene Co., Ltd. (8/08), Takara Bio Inc. (8/08), Roche Applied Science (8/08), New England Biolabs (8/08), Toyobo Biochemicals (8/08), Molecular Biology Resources - CHIMERx (8/08), Promega Corporation (8/08), Sigma Chemical Corporation (8/08), Bangalore Genei (8/08), Vivantis Technologies (8/08), MP Biomedicals (8/08), EURx Ltd. (8/08), and CinnaGen Inc. (8/08). PmeI_Isoschizomer with MssI. Available from New England Biolabs (8/08), and MP Biomedicals (8/08). 44First GenerationpcDNA3.1(Invitrogen)0Toolbox sequenced area from position 838-1631. Standard Codon Table     k    () *?Vr )((53.121$$