LOCUS pmCherry-N1-Crk 5632 bp DNA circular COMMENT The vendor of vector is Clontech, www.clontech.com; cat# 632523. Toolbox sequenced area from position 480 - 1438 and 1474 - 2430. DEFINITION The vendor of vector is Clontech, www.clontech.com; cat# 632523. Toolbox sequenced area from position 480 - 1438 and 1474 - 2430. FEATURES Location/Qualifiers misc_feature 1..589 /label=CMV_IE CDS 656..2299 /label= mCherry-N1-Crk misc_feature 656..1567 /label= hCrk /note=" >ref|NP_058431.2| adapter molecule crk isoform a [Homo sapiens]" misc_feature complement(2548..2983) /label= f1 ori misc_feature 1589..2299 /label= mCherry /note=" human codon optimized;mCherry is a mutant fluorescent protein derived from the tetrameric red fluorescent protein DsRed for further information see on the Clontech website: http://www.clontech.com/support/vectors.asp?cat_id=632523&q=&image.x=39&image.y=10" misc_feature 4907..5550 /label= pUC ori CDS complement(3528..4322) /label= kan/neo R /note="Neomycin phosphotransferase coding sequences, kanamycin resistance" ORIGIN 1 TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGG 186 TGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATG 371 CGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTA 556 TATAAGCAGAGCTGGTTTAGTGAACCGTCAGATCCGCTAGCGCTACCGGACTCAGATCTCGAGCTCAAGCTTCGAATTCTGCAGTCGACGGTACcccacc ATG GCG GGC AAC TTC GAC TCG GAG GAG CGG AGT AGC TGG TAC TGG GGG CGG TTG AGT CGG CAG 719 GAG GCG GTG GCG CTG CTG CAG GGC CAG CGG CAC GGG GTG TTC CTG GTG CGG GAC TCG AGC ACC AGC CCC GGG GAC TAT GTG CTC AGC GTC TCA GAG AAC TCG CGC GTC TCC CAC TAC ATC ATC AAC AGC AGC GGC CCG 857 CGC CCG CCG GTG CCA CCG TCG CCC GCC CAG CCT CCG CCC GGG GTG AGC CCC TCC AGA CTC CGA ATA GGA GAT CAA GAG TTT GAT TCA TTG CCT GCT TTA CTG GAA TTC TAC AAA ATA CAC TAT TTG GAC ACT ACA ACG 995 TTG ATA GAA CCA GTT TCC AGA TCC AGG CAG GGT AGT GGA GTG ATT CTC AGG CAG GAG GAG GCG GAG TAT GTG CGA GCC CTC TTT GAC TTT AAT GGG AAT GAT GAG GAA GAT CTT CCC TTT AAG AAA GGA GAC ATC TTG 1133 AGA ATC CGG GAC AAG CCT GAA GAG CAG TGG TGG AAT GCG GAG GAC AGC GAA GGC AAG AGA GGG ATG ATT CCA GTC CCT TAC GTC GAG AAG TAT AGA CCT GCC TCC GCC TCA GTA TCG GCT CTG ATT GGA GGT AAC CAG 1271 GAG GGT TCC CAC CCA CAG CCA CTG GGT GGG CCG GAG CCT GGG CCC TAT GCC CAA CCC AGC GTC AAC ACT CCG CTC CCT AAC CTC CAG AAT GGG CCC ATA TAT GCC AGG GTT ATC CAG AAG CGA GTC CCC AAT GCC TAC 1409 GAC AAG ACA GCC TTG GCT TTG GAG GTC GGT GAG CTG GTA AAG GTT ACG AAG ATT AAT GTG AGT GGT CAG TGG GAA GGG GAG TGT AAT GGC AAA CGA GGT CAC TTC CCA TTC ACA CAT GTC CGT CTG CTG GAT CAA CAG 1547 AAT CCC GAT GAG GAC TTC AGC GGG GAT CCA CCG GTC GCC ACC ATG GTG AGC AAG GGC GAG GAG GAT AAC ATG GCC ATC ATC AAG GAG TTC ATG CGC TTC AAG GTG CAC ATG GAG GGC TCC GTG AAC GGC CAC GAG TTC 1685 GAG ATC GAG GGC GAG GGC GAG GGC CGC CCC TAC GAG GGC ACC CAG ACC GCC AAG CTG AAG GTG ACC AAG GGT GGC CCC CTG CCC TTC GCC TGG GAC ATC CTG TCC CCT CAG TTC ATG TAC GGC TCC AAG GCC TAC GTG 1823 AAG CAC CCC GCC GAC ATC CCC GAC TAC TTG AAG CTG TCC TTC CCC GAG GGC TTC AAG TGG GAG CGC GTG ATG AAC TTC GAG GAC GGC GGC GTG GTG ACC GTG ACC CAG GAC TCC TCC CTG CAG GAC GGC GAG TTC ATC 1961 TAC AAG GTG AAG CTG CGC GGC ACC AAC TTC CCC TCC GAC GGC CCC GTA ATG CAG AAG AAG ACC ATG GGC TGG GAG GCC TCC TCC GAG CGG ATG TAC CCC GAG GAC GGC GCC CTG AAG GGC GAG ATC AAG CAG AGG CTG 2099 AAG CTG AAG GAC GGC GGC CAC TAC GAC GCT GAG GTC AAG ACC ACC TAC AAG GCC AAG AAG CCC GTG CAG CTG CCC GGC GCC TAC AAC GTC AAC ATC AAG TTG GAC ATC ACC TCC CAC AAC GAG GAC TAC ACC ATC GTG 2237 GAA CAG TAC GAA CGC GCC GAG GGC CGC CAC TCC ACC GGC GGC ATG GAC GAG CTG TAC AAG TAG CGGCCGCGACTCTAGATCATAATCAGCCATACCACATTTGTAGAGGTTTTACTTGCTTTAAAAAACCTCCCACACCTCCCCCTGAACCTGAAACATAAAAT 2401 GAATGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTAAGGCGTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTA 2586 AATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACT 2771 ACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGC 2956 GCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTCCTGAG 3141 GCGGAAAGAACCAGCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTC 3326 CCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAGATCGATCAAGAGACAG 3511 GATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCC 3696 CTGAATGAACTGCAAGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGA 3881 TGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGAGCATGCCCG 4066 ACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTC 4251 GTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCTGCCATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGG 4436 GACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCACCCTAGGGGGAGGCTAACTGAAACACGGAAGGAGACAATACCGGAAGGAACCCGCGCTATGACGGCAATAAAAAGACAGAATAAAACGCACGGTGTTGGGTCGTTTGTTCATAAACGCGGGGTTCGGTC 4621 CCAGGGCTGGCACTCTGTCGATACCCCACCGAGACCCCATTGGGGCCAATACGCCCGCGTTTCTTCCTTTTCCCCACCCCACCCCCCAAGTTCGGGTGAAGGCCCAGGGCTCGCAGCCAACGTCGGGGCGGCAGGCCCTGCCATAGCCTCAGGTTACTCATATATACTTTAGATTGATTTAAAAC 4806 TTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCA 4991 GCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAG 5176 TGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGG 5361 ACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGG 5546 TTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCATGCAT //