Ŗ¦gggcgaattaattcgagctcggtacccagcttgcttgttctttttgcagaagctcagaataaacgctcaactttggcagatcaattccccggggatccgtcgaccatgaagaaagtgattatcaatacatggcgctcttttgtaaatattcccaatgtggttggaccagatgttgaaaaggaaataacaaggatggaaaatggagcatgcagctccttttctgatgatgatgatgacagtgcctctatgtttgaagaatcaggaaatgagaacccccacgcaacggattcctttagaagtaatacacacggaagtggacaaccatcacagagggagcaatacctgcctggagccattgcactttttaatgttaacgagacggagcagcggcccgactcgtttcttgagcgattccgtggaccccaggctgcaaacgatcaatcagctgcccccgcagacgcccccaagaaaaccttcaaggagaggtgggaaggctttgtcgtgtcccagtctgatgatatttattattattggctgttcttcatcgccttggcttcactctataactggatcatgctggttgccagagcgtgtttcgatcaactacaggacgagaacttcttcctgtgggtggggctggattacctctgcgatgtcatttacatactggacacctgtatacgattgaggacaggatacctcgagcaaggtctgctggtgaaagacttggccaagttgagagacaactacattcgcacttttcaatttaaacttgatgttctatcagtgatcccaactgatctgctgtatattaagtttggctggaattatccagaaattagattaaacagattgttaaggatctctcgaatgtttgagttcttccagagaacagaaacacggacaaactacccaaacatcttcaggatctctaaccttgtcatgtacatcatcatcatcatccactggaatgcgtgtgtgtacttctctatttccaaagctattgggtttggaaatgacacatgggtctatcctgatgttaatgatcctgattttggccgtttgtcgttctgctatgtgtactgcttctactggtccacactaacactaacaaccattggggaaatgccccctccagtgaaggatgaggagtatgtctttgtagtttttgatttcctcattggagtgttaatttttgccaccattgtcggtaacataggttctatgatttccaacatgaatgcggccagggcagaatttcaagcaagaattgatgcaatcaagcaatacatgcattttcgaaatgtaagcaaagatatggagaagcgagttattaaatggtttgactatctgtggaccaacaaaaaaacagtggatgagagagaagtcttgaagtatctacctgataaactaagagcagagatcgccatcaatgttcatttagacacattaaaaaaggtgcgcatttttgcagactgtgaagccggcctgctggaggaactggtgctcaagctcaccccacaggtctacaaccctggagagtacgtgtgtaggaaaggtgacgtagggcatgagatgtacatcattaaggaggggaagttggctgtggtggcagacgatggggtcactcagtttgctgtactgggtgacgggaacttctttggagagatcagcattctcaacataaaaggcaacaaatcaggcaatcggcgcacggctaacatccgcagcatcggttactcagaccttttcagcctgtccaaggaggacctgacagacactctgtctgagttcccagctgccaagcgccttctggaggaaaagggacgccagatcctcaccaagatgggaatgctagagatttcggatgaaggggaggctggaagtgatcctaaagatcttgaagagaaggtcacccgaatggagagctcagtagacctcctgcaaacaaggtttgctcgcatcctggctgagtatgagtcaatgcagcagaaactgaagcagaggctaaccaaggttgagaaattcctgaaaccacttattgacacagaattctcagctattgaaggatctggaactgaaagtgggcccacagactctacacaggacactagtggtggcagcggaggcagccgcggatctggaggtagtggcggaatggtgagcaagggcgaggaggataacatggccatcattaaggagttcatgcgcttcaaggtgcacatggaggggtccgtgaacggccacgtgttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggcggccccctgcccttcacctgggacatcctgagccctcagttcatgtacggctccaatgcctacgtgaagcaccccgccgacatccccgactactttaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaaattcgaggacggcggcgtggtgaccgtgacccaggactcctccctgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtgatgcagaagaagaccatgggctgggaggccctctccgagcggatgtaccccgaggacggcgccctgaagggcgaggtcaagcccagagtgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaaccgcaagctggacatcaccagccacaacgaggactacaccatcgtggagcagtacgagagagccgagggccgccactccaccggcggcatggacgagctgtacaagtaaagcggccgcgacactagtgcaagcttgatctggttaccactaaaccagcctcaagaacacccgaatggagtctctaagctacataataccaacttacactttacaaaatgttgtcccccaaaatgtagccattcgtatctgctcctaataaaaagaaagtttcttcacattctaaaaaaaaaaaaaaaaaaaaaaaccccccccccccccccccccccccccccccctgcaggcatgcaagctagcttgagtattctatagtgtcacctaaatagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttcgataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcaatgctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatagtaccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttggcattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatcggtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataatagtgtatgacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtgagcgggtgttggcgggtgtcggggctggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgaattgtaatacgactcactataā¦i† ŻkĀ¢’’’’’’’’i=† Ō’’’’’’’’=m† ’’’’’’’’m 4† €°’’’’’’’’ 4¦† ŻkĀ¢’’’’’’’’²¦ i 4 ŃPŖUŖUŖUŖU‹ ¬$Œ‹i= ŖUŖUŖUŖU‹ ¬&Z¬&L‹(… ³dm 4 ŖUŖUŖUŖU‹ ¬&v¬&h‹(žė,Š’’’Q 1 E ŖUŖUŖUŖU‹ } ¬¬#f‹Fuj°‡•© ŖUŖUŖUŖU‹ «R<«R ‹Žß²’’’„ ¦E ŖUŖUŖUŖU‹ «Rf«RJ‹·ĮŪ1ā< ŃpŖUŖUŖUŖU‹ «Rt‹i©ūW ŖUŖUŖUŖU‹ «Rž«R‹©÷ĶW• H  ŖUŖUŖUŖU‹ «Rŗ«R¬‹KõoU’’žŽ=m ŖUŖUŖUŖU‹ «Rȋ̢𒒒ęXfA4-PAmCherry1 fusion proteinXfA4-Xfa4-Channel (modified chimeric CNG-Channel) PAmCherry1w>gb|AY678264.1| Synthetic construct monomeric red fluorescent protein gene mCherry; engineered variant of monomeric red fluorescent protein mRFP1 in GenBank Accession Number AF506027; N- and C-termini replaced with equivalent residues from EGFP PMID: 19169259;PAmCherry1 with excitation/emission maxima at 564/595 nm derived from mCherry (product of Clontech); "Lack of green fluorescence and single-molecule behavior make monomeric PAmCherry1 a preferred tag for two-color diffraction-limited photoactivation imaging and for super-resolution techniques such as one- and two-color photoactivated localization microscopy (PALM)."SP6primerT7T7 primer/ promoterpBR322+ori derived from pBR322 of Escherichia coliampR5' UTR!5' UTR of a Xenopus 8-globin gene3' UTR!3' UTR of a Xenopus 8-globin geneLinker 2&¦ W]DW‹¦ł ’ž‡‹ () (Pø°¤÷\bD\‹¦ł’æ>ŽVŌ ()T(°ø°¤÷bh$‹«Rä«RÖ’Ø?‘aÉ ()“(裤÷=CD= ‹¦ł0 žø¾ēÜÓ () É.ø°¤÷TZDT ‹¦ł>žŖ¾Ņ˜/ ().ÉR ø°¤÷ 5 =D 5‹¦ł„’«æ»qĻ ()ņ«āø°¤÷ < H D <‹¦ł¼’ĮæP¶Ō ()ņO§ø°¤÷ < H D <‹¦ł® ’ƽdæŹ ()ņóKø°¤÷ @ FD @‹¦ś, ’Å?… ()ņ±ļø°¤÷ H N$‹«S«Rņ’¾?£T“ ()ņW­£¤÷,8 D, ‹¬‰žÜ¾ŚAć ()@\ø°¤÷,8 D, ‹¬‰ žŠ¾ÆYą ()ų\ø°¤÷SmaIBamHISalIIsoschizomer with Acs1371I, Acs1372I, Acs1373I, Acs1421I, Acs1422I, BspMKI, BstZ16I, BtgAI, CglAII, HgiCIII, HgiDII, KoyI, NopI, Psp32I, Psp33I, Psp89I, RflFI, RheI, RhpI, RrhI, Rrh4273I, RroI, RtrI, Rtr63I, Uba89I, XamI, and XciI. Available from Invitrogen Corporation (8/08), Minotech Biotechnology (8/08), Fermentas International Inc. (8/08), American Allied Biochemical, Inc. (8/08), SibEnzyme Ltd. (8/08), Nippon Gene Co., Ltd. (8/08), Takara Bio Inc. (8/08), Roche Applied Science (8/08), New England Biolabs (8/08), Toyobo Biochemicals (8/08), Molecular Biology Resources - CHIMERx (8/08), Promega Corporation (8/08), Sigma Chemical Corporation (8/08), Bangalore Genei (8/08), Vivantis Technologies (8/08), MP Biomedicals (8/08), EURx Ltd. (8/08), and CinnaGen Inc. (8/08). SpeISacIINotIBcgI-1BcgI-2SpeIHindIIIhIsoschizomer with Asp52I, Asp3065I, BbrI, BbrAI, BpeI, BseHI, BsmGII, BspKT8I, BspLAIII, Bst170II, BstFI, BstZ1II, Cfr32I, ChuI, Csp231I, Eco65I, Eco98I, Eco188I, Eco231I, EcoVIII, Hin5III, Hin173I, Hin1076III, HinJCII, HinSAFI, HinbIII, HinfII, HsuI, LlaCI, MkiI, SpaPIV, SsbI, Uba83I, Uba1164II, Uba1219I, and Uba1435I. Available from Invitrogen Corporation (8/08), Minotech Biotechnology (8/08), Fermentas International Inc. (8/08), American Allied Biochemical, Inc. (8/08), SibEnzyme Ltd. (8/08), Nippon Gene Co., Ltd. (8/08), Takara Bio Inc. (8/08), Roche Applied Science (8/08), New England Biolabs (8/08), Toyobo Biochemicals (8/08), Molecular Biology Resources - CHIMERx (8/08), Promega Corporation (8/08), Sigma Chemical Corporation (8/08), Bangalore Genei (8/08), Vivantis Technologies (8/08), MP Biomedicals (8/08), EURx Ltd. (8/08), and CinnaGen Inc. (8/08). BcgI-1BcgI-2¦¦0 (Most Recent Generation) ’ō () ’ō ()pGEMHE-XfA4-PAmCherry1)Toolbox sequenced area from pos. 27-2112.bbStandard Codon Table      ’’’’’’’’€°‹ ‹ŖUŖUŖUŖU‹  ()† *ž?€ Ž<((63.6488882)$$’’’Ü’’’Ü