LOCUS pCMV_EGFP-µMHCTM. 5667bp DNA circular COMMENT Toolbox sequenced area from position 515-3230. DEFINITION Toolbox sequenced area from position 515-3230. FEATURES Location/Qualifiers misc_feature 30..472 /label=CMV immearly promoter misc_feature 88..374 /label=CAG enhancer misc_feature 542..584 /label=CMV promoter CDS 809..2818 /label= EGFP-µMHCTM misc_feature 887..1603 /label= EGFP misc_feature 1631..2587 /label=mIgM_heavy_chain /note=" >gb|AAC08349.1| linked to AAC08349.1 immunoglobulin heavy chain [Mus musculus]; CH3+CH4 domains of Immunglobulin domain of the heavy chain of IgM" misc_feature 2708..2818 /label=MHCI_tm /note=" MHC I transmembrane region" misc_feature 2919..3196 /label=BGH polyAsite /note=" polyA site of bovine growth hormone (BGH)" CDS complement(4443..5303) /label= ampR /note="ß-lactamase" misc_feature complement(3242..3261) /label=T3 misc_feature 635..654 /label=T7 misc_feature 3669..4288 /label= pUC ori /note="pUC ori is equivalent to pBR322 ori; the origin of replication (ori) was derived originally from a ColE1-like plasmid, pMB1" ORIGIN 1 CGCGTTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTC 81 CGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTA 161 TGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGACTATTTACGGTAAACTGCCCACTTGGCAG 241 TACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAG 321 TACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTG 401 GCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTT 481 TGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGT 561 GTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACCCACTGCTTACTGGCTTATCGAAATTAATAC 641 GACTCACTATAGGGAGACCCAAGCTTCGAATTCTGCAGTCGACGGTACCGGGCCCCCCCTCGAGGTCGACCTACGTAACC 721 ATGGGATGGAGCTGTATCATGCTCTTCTTGGCAGCAACAGCTACAGGTAAGGGGCTCACAGTAGCAGGCTTGAGGTCTGG 801 ACATATAC ATG GGT GAC AAT GAC ATC CAC TTT GCC TTT CTC TCC ACA GGT GTC CAC TCC CAG 863 GTC CAA CTG CAG CCC GGG GGA TCC ATG GTG AGC AAG GGC GAG GAG CTG TTC ACC GGG GTG 923 GTG CCC ATC CTG GTC GAG CTG GAC GGC GAC GTA AAC GGC CAC AAG TTC AGC GTG TCC GGC 983 GAG GGC GAG GGC GAT GCC ACC TAC GGC AAG CTG ACC CTG AAG TTC ATC TGC ACC ACC GGC 1043 AAG CTG CCC GTG CCC TGG CCC ACC CTC GTG ACC ACC CTG ACC TAC GGC GTG CAG TGC TTC 1103 AGC CGC TAC CCC GAC CAC ATG AAG CAG CAC GAC TTC TTC AAG TCC GCC ATG CCC GAA GGC 1163 TAC GTC CAG GAG CGC ACC ATC TTC TTC AAG GAC GAC GGC AAC TAC AAG ACC CGC GCC GAG 1223 GTG AAG TTC GAG GGC GAC ACC CTG GTG AAC CGC ATC GAG CTG AAG GGC ATC GAC TTC AAG 1283 GAG GAC GGC AAC ATC CTG GGG CAC AAG CTG GAG TAC AAC TAC AAC AGC CAC AAC GTC TAT 1343 ATC ATG GCC GAC AAG CAG AAG AAC GGC ATC AAG GTG AAC TTC AAG ATC CGC CAC AAC ATC 1403 GAG GAC GGC AGC GTG CAG CTC GCC GAC CAC TAC CAG CAG AAC ACC CCC ATC GGC GAC GGC 1463 CCC GTG CTG CTG CCC GAC AAC CAC TAC CTG AGC ACC CAG TCC GCC CTG AGC AAA GAC CCC 1523 AAC GAG AAG CGC GAT CAC ATG GTC CTG CTG GAG TTC GTG ACC GCC GCC GGG ATC ACT CTC 1583 GGC ATG GAC GAG CTG TAC AAG TCC GGA CTC AGA TCT CGA GCT CAA GCT GAT CTG CAT GTG 1643 CCC ATT CCA GCT GTC GCA GAG ATG AAC CCC AAT GTA AAT GTG TTC GTC CCA CCA CGG GAT 1703 GGC TTC TCT GGC CCT GCA CCA CGC AAG TCT AAA CTC ATC TGC GAG GCC ACG AAC TTC ACT 1763 CCA AAA CCG ATC ACA GTA TCC TGG CTA AAG GAT GGG AAG CTC GTG GAA TCT GGC TTC ACC 1823 ACA GAT CCG GTG ACC ATC GAG AAC AAA GGA TCC ACA CCC CAA ACC TAC AAG GTC ATA AGC 1883 ACA CTT ACC ATC TCT GAA ATC GAC TGG CTG AAC CTG AAT GTG TAC ACC TGC CGT GTG GAT 1943 CAC AGG GGT CTC ACC TTC TTG AAG AAC GTG TCC TCC ACA TGT GCT GCC AGT CCC TCC ACA 2003 GAC ATC CTA ACC TTC ACC ATC CCC CCC TCC TTT GCC GAC ATC TTC CTC AGC AAG TCC GCT 2063 AAC CTG ACC TGT CTG GTC TCA AAC CTG GCA ACC TAT GAA AC T CTG AAT ATC TCC TGG GCT 2123 TCT CAA AGT GGT GAA CCA CTG GAA ACC AAA ATT AAA ATC ATG GAA AGC CAT CCC AAT GGC 2183 ACC TTC AGT GCT AAG GGT GTG GCT AGT GTT TGT GTG GAA GAC TGG AAT AAC AGG AAG GAA 2243 TTT GTG TGT ACT GTG ACT CAC AGG GAT CTG CCT TCa CCA CAG AAG AAA TTC ATC TCA AAA 2303 CCC AAT GAG GTG CAC AAA CAT CCA CCT GCT GTG TAC CTG CTG CCA CCA GCT CGT GAG CAA 2363 CTG AAC CTG AGG GAG TCA GCC ACA GTC ACC TGC CTG GTG AAG GGC TTC TCT CCT GCA GAC 2423 ATC AGT GTG CAG TGG CTT CAG AGA GGG CAA CTC TTG CCC CAA GAG AAG TAT GTG ACC AGT 2483 GCC CCG ATG CCA GAG CCT GGG GCC CCA GGC TTC TAC TTT ACC CAC AGC ATC CTG ACT GTG 2543 ACA GAG GAG GAA TGG AAC TCC GGA GAG ACC TAT ACC TGT GTT GTA GAA CAT GAG GCC TCA 2603 AAG ACA AAG CTT AAT GCC AGC AAG AGC CTA GCA ATT AGT GGC ATA GTC AAC ACC ATC CAA 2663 CAC TCG TGT ATC ATG GAT GAG CAA AGT GAC AGC TAC ATG GAC CTC GAG CCT CCT CCA TCC 2723 ACT GTC TCC AAC ACG GTA ATC ATT GCT GTT CTG GTT GTC CTT GGA GCT GCA ATA GTC ACT 2783 GGA GCT GTG GTG GCT TTT GTG ATG AAG GTG AAG TAG ATCAAGCTTATCGATCCAGATCCAGACATGAT 2851 AAGATACATTGATGAGTTTGGACAAACCATCGATCTCGACCTGCAGGAATTCGATATCTGCGGCCGCTCTAGAGCTCGCT 2931 GATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGT 3011 GCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGG 3091 TGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGCTT 3171 CTGAGGCGGAAAGAACCAGCTGGGGCTCGAGCCCGGGCGCGCCAAGCTTAATTAAGTACCCAGCTTTTGTTCCCTTTAGT 3251 GAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACAC 3331 AACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTC 3411 ACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC 3491 GTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCAC 3571 TCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGC 3651 CAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCT 3731 CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCT 3811 GTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTG 3891 TAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCG 3971 CCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGG 4051 ATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGT 4131 ATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCG 4211 CTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTT 4291 TCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCAC 4371 CTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAG TTA CCA 4449 ATG CTT AAT CAG TGA GGC ACC TAT CTC AGC GAT CTG TCT ATT TCG TTC ATC CAT AGT TGC 4509 CTG ACT CCC CGT CGT GTA GAT AAC TAC GAT ACG GGA GGG CTT ACC ATC TGG CCC CAG TGC 4569 TGC AAT GAT ACC GCG AGA CCC ACG CTC ACC GGC TCC AGA TTT ATC AGC AAT AAA CCA GCC 4629 AGC CGG AAG GGC CGA GCG CAG AAG TGG TCC TGC AAC TTT ATC CGC CTC CAT CCA GTC TAT 4689 TAA TTG TTG CCG GGA AGC TAG AGT AAG TAG TTC GCC AGT TAA TAG TTT GCG CAA CGT TGT 4749 TGC CAT TGC TAC AGG CAT CGT GGT GTC ACG CTC GTC GTT TGG TAT GGC TTC ATT CAG CTC 4809 CGG TTC CCA ACG ATC AAG GCG AGT TAC ATG ATC CCC CAT GTT GTG CAA AAA AGC GGT TAG 4869 CTC CTT CGG TCC TCC GAT CGT TGT CAG AAG TAA GTT GGC CGC AGT GTT ATC ACT CAT GGT 4929 TAT GGC AGC ACT GCA TAA TTC TCT TAC TGT CAT GCC ATC CGT AAG ATG CTT TTC TGT GAC 4989 TGG TGA GTA CTC AAC CAA GTC ATT CTG AGA ATA GTG TAT GCG GCG ACC GAG TTG CTC TTG 5049 CCC GGC GTC AAT ACG GGA TAA TAC CGC GCC ACA TAG CAG AAC TTT AAA AGT GCT CAT CAT 5109 TGG AAA ACG TTC TTC GGG GCG AAA ACT CTC AAG GAT CTT ACC GCT GTT GAG ATC CAG TTC 5169 GAT GTA ACC CAC TCG TGC ACC CAA CTG ATC TTC AGC ATC TTT TAC TTT CAC CAG CGT TTC 5229 TGG GTG AGC AAA AAC AGG AAG GCA AAA TGC CGC AAA AAA GGG AAT AAG GGC GAC ACG GAA 5289 ATG TTG AAT ACT CAT ACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATA 5364 CATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCGACG 5444 GATCGGGAGATCTCCCGATCCCCTATGGTCGACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATCTGC 5524 TCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAAT 5604 TGCATGAAGAATCTGCTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATA //